Abstract
Abstract Soybean products are popular because of its taste, digestibility, and health benefits. However, soybean lacks vitamin, mainly the low water-soluble vitamin B12. This study investigated the effects of fermentation conditions on the synthesis of vitamin B12, production of metabolites, and growth of Lactobacillus reuteri and Propionibacterium shermainii in fermented soy-milk. A Lotka Volterra model was successfully employed to describe the competition relationship between the two microorganisms under various fermentation conditions. A quadratic function between the ratio of interaction coefficients and vitamin B12 content was found. Higher vitamin B12 in soy-milk can be produced when the ratio of interaction coefficients approach to one. Compared with other fermented soybean products, fermented soy-milk contains more acetate, ethanol, and propionic acid. This study successfully demonstrated a mathematical model to enhance soy-milk vitamin B12 production.
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