Enhancing the treatment performance of partial denitrification/Anammox process at high nitrogen load: Effects of immobilized strain HFQ8C/N on the sludge characteristics

Abstract

A novel strategy based on quorum sense (QS) was proposed to improve the treatment performance of the partial denitrification/Anammox (PD/A) process at high loads by adding immobilized Pseudomonas sp. HFQ8C/N, which could release high concentrations of N-butyryl-DL-homoserine lactone (C4-HSL), N-octanoyl-DL- homoserine lactone (C8-HSL) and N-decanoyl-DL-homoserine lactone (C10-HSL). The results showed that adding immobilized HFQ8C/N improved the sludge activity and settleability, contributing to higher nitrogen removal efficiency at the high nitrogen loading rate (NLR). It was proved that C4-HSL promoted the abundances of Thauera and Candidatus Kuenenia at NLR 1.68–2.52 kg N/(m3·d), while C10-HSL promoted the abundance of Candidatus Brocadia. Besides, C8-HSL and C10-HSL played different regulation roles in the production of protein (PN) in tightly bound extracellular polymeric substances (TB-EPS) at different loads, improving the sludge settleability. This study provided a new way to improve the treatment performance of high-load PD/A processes.