Abstract
Accurate determination of cysteic acid, homocysteic acid, and taurine in aqueous solution is essential in many analyses of biological and clinical applications. These sulfonic acids are difficult to be separated and determined using reversed phase chromatography and cation‐exchange chromatography. In this research, an accurate and sensitive determination for the three sulfonic acids has been achieved using anion‐exchange chromatography with post‐column derivatization and spectrometric detection. This technique has outperformed the technique using suppressed conductivity detection. It has enormously enhanced the accuracy, detection limit, sensitivity, and linearity in the determination of these three sulfonic acids. This technique has demonstrated to be excellent for simultaneously determining cysteic acid, homocysteic acid, and taurine.
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