Abstract
Accurate determination of low molecular weight sulfonic acids including cysteic acid, homocysteic acid, and taurine in aqueous solution is essential in many analyses of biological and clinical applications. These sulfonic acids are difficult to be separated and determined using reversed phase chromatography and cation‐exchange chromatography. In this research, anion‐exchange chromatography with suppressed conductivity detection is utilized in the separation and determination of these compounds. This technique has achieved excellent separation for these sulfonic acids. Among the three sulfonic acids, the calibration for taurine has demonstrated the poorest accuracy, the lowest sensitivity, narrowest linearity, and poorest limit of detection, as well as reproducibility. The inadequacy of this technique to quantify taurine is due to the low conductivity of taurine in aqueous solution. The characteristics of calibration curves for homocysteic acid and cysteic acid are exceptionally perfect. This technique has demonstrated to be excellent for accurately determining cysteic acid and homocysteic acid.
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