Enhancing effects of heat shock and gibberellic acid on the thermotolerance in etiolated Vigna radiata. I. Physiological aspects on thermotolerance

Abstract

The effect of heat shock on the thermotolerance of etiolated mung bean seedlings (Vigna radiata L. cv. Wilczek) and the effects of gibberellic acid (GA) were studied. The potentially lethal temperature of etiolated mung bean seedlings was 45°C. But, when seedlings were pretreated with a heat‐shock period at 40°C for 1 h before incubation at 45°C, they become thermotolerant and survived the 45°C treatment. The addition of actinomycin D or cycloheximide during the heat‐shock period decreased the subsequent thermotolerance of the seedlings. Depending upon the time of its application, GA appeared to have multiple effects: (1) when applied during the 40°C heat‐shock period, GA enhanced the heat‐shock effect; (2) when applied during the 45°C potentially lethal temperature period, GA enhanced the subsequent growth of hypocotyls. This suggests that GA makes the seedlings tolerant to the potentially lethal temperature; (3) when GA was applied during a following 25°C growth period to seedlings which had been exposed first to 40°C and then 45°C, it promoted growth, suggesting that GA enhanced the restoration of the seedlings from high temperature damage. The role of GA and heat shock in the acquisition of thermotolerance in etiolated mung bean seedlings are discussed.