Abstract
The combination of combined fulvic acid (FA) and two-step cultivation was proposed as a novel strategy to enhance biomass and oil accumulation of Monoraphidium sp. FXY-10 compared with the traditional monoculture of microalgae. In this study, the effect of FA on the growth and lipid accumulation of Monoraphidium sp. FXY-10 was investigated using one- and two-stage cultivation. In the heterotrophic one-stage cultivation, 1.5-fold biomass was enhanced with 80mg L−1 FA (6.41g L−1) compared with the control biomass (4.21g L−1). In the heterotrophic–photoautotrophic two-stage cultivation, different FA concentrations were used to stimulate lipid accumulation. The lipid content increased from 30.78% to 54.65% when 25mg L−1 FA was added to the culture. The main fatty acids in the FA-triggered Monoraphidium sp. FXY-10 were C16/C18 fatty acids (97%), which are appropriate for biodiesel production. Results showed that the proposed strategy is useful for biomass and oil production of Monoraphidium sp. FXY-10.
Published Version
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