Abstract
In this work, a novel pretreatment strategy through combination of ball-milling and LiCl/DMSO dissolution and regeneration was developed, and its effect on bagasse enzymatic hydrolysis was investigated. The results showed that, after ball-milling, the bagasse crystallinity and average particle size were declined from 48.1% to 22.6% and 118.6 μm–20.7 μm, respectively, however, its surface area was augmented from 4.9 m2/g to 10.3 m2/g. Following LiCl/DMSO dissolution and regeneration, a certain amount of lignin (∼26.3%) was removed, and portion of cellulose crystalline structure were swelled and converted from type I to II. The highest yields of glucose and xylose obtained from above combined pretreatment were 87.8% and 84.2%, respectively, which were both significantly higher than those obtained from single ball-milling or LiCl/DMSO dissolution and regeneration. It can be concluded that the combination of ball-milling and LiCl/DMSO dissolution and regeneration was a promising approach for improving bagasse enzymatic saccharification.
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