Abstract
Candida cylindracea lipase was entrapped in methyl-substituted organic silicates formed on kieselguhr such as Celite 545 and Hyflo Super-Cel. Various preparation conditions were optimized to obtain a more thermostable immobilized lipase. The hybrid gel-entrapped lipase derived from an equimolar mixture of trimethylmethoxysilane and tetramethoxysilane, retained its full activity for ester synthesis up to the temperatures of 65°C.
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