Enhancement of Polyphenols Recovery from Rosa canina, Calendula officinalis and Castanea sativa Using Pulsed Electric Field

Achillia Lakka,Stavros I Lalas,Eleni Bozinou,Vassilis Athanasiadis,Iordanis Samanidis,Dimitris P Makris,Giorgos Stavropoulos,Vassilis G Dourtoglou

doi:10.3390/beverages7030063

Achillia Lakka, Stavros I Lalas + Show 6 more

Open Access

https://doi.org/10.3390/beverages7030063

Copy DOI

Journal: Beverages	Publication Date: Sep 3, 2021
Citations: 12	License type: CC BY 4.0

Affiliation: University of Thessaly, University of West Attica

Abstract

The current study evaluates the Pulsed Electric Field (PEF) technique for the extraction of polyphenols from the plants Rosa canina, Calendulaofficinalis and Castanea sativa. These plants are traditionally used both for the preparation of therapeutic decoctions and the aromatization of beverages (alcoholic or not). Pulses of 10 μs duration were used to apply electric field intensities ranging from 1.2 to 2.0 kV cm−1. The period of the phenomenon was set to 1 ms, with a total extraction time of 20 min. The total polyphenol content as well as the identified polyphenolic compounds of the extracts were determined for monitoring and evaluation. To estimate the PEF effect, control extracts were prepared using the same process as PEF extracts but without the application of electric field. For all the three plant materials studied, the PEF technique appeared to be successful in increasing polyphenols extraction. The application of a moderate to high electric field, up to 1.4 kV cm−1, resulted in increased total and individual polyphenols recovery, reaching 63.79% and 84%, respectively, in the case of Rosa canina fruits.

Highlights

Medicinal plants comprise a variety of pharmaceutical properties due to the presence of various bioactive phytoconstituents that possess distinct mechanisms of action.Those bioactive phytoconstituents could further be utilized as potential alternatives to conventional drugs, a field that is gaining an important position and great demand globally as herbal formulations are safer, cheaper, and have negligible side effects, compared to their synthetic analogs [1,2].The Swiss physician and chemist Paracelsus (1493–1541) expressed the basic principle of toxicology: Natural substances are the best healers, but all substances are poison, and it is only the correct dose which makes them a remedy [3]
It can be concluded that Pulsed Electric Field (PEF) is a very promising technique in the field of bioactive compounds extraction from plants that could offset several of the disadvantages of conventional extraction methods
In comparison to the control samples, PEF treatment of Rosa canina fruits, Calendula officinalis flowers and Castanea sativa leaves resulted in extracts with increased polyphenol content

Summary

Introduction

Medicinal plants comprise a variety of pharmaceutical properties due to the presence of various bioactive phytoconstituents that possess distinct mechanisms of action.Those bioactive phytoconstituents could further be utilized as potential alternatives to conventional drugs, a field that is gaining an important position and great demand globally as herbal formulations are safer, cheaper, and have negligible side effects, compared to their synthetic analogs [1,2].The Swiss physician and chemist Paracelsus (1493–1541) expressed the basic principle of toxicology: Natural substances are the best healers, but all substances are poison, and it is only the correct dose which makes them a remedy [3]. Medicinal plants comprise a variety of pharmaceutical properties due to the presence of various bioactive phytoconstituents that possess distinct mechanisms of action. Those bioactive phytoconstituents could further be utilized as potential alternatives to conventional drugs, a field that is gaining an important position and great demand globally as herbal formulations are safer, cheaper, and have negligible side effects, compared to their synthetic analogs [1,2]. Herbal products pose a number of risks (e.g., toxins, pesticides, heavy metals, alkaloids, saponins, etc.) [4,5,6,7], but other functional components having useful actions (i.e., antioxidant activity) show a great potential for illness prevention and treatment, as well as human health promotion [8]. Consumers tend to favor the use of additives of natural origin attributing to artificial additives of various suspected undesirable actions of such as the promotion of carcinogenesis or mutagenicity [9]

Objectives

Methods

Results

Conclusion