Abstract
Strain improvement through random mutagenesis is an extremely developed practice and it plays an important role in the economic growth of microbial agitation processes. The present study comprises of genetic improvement of fungus isolated from petrol pump soil by ethyl methane sulfonate (EMS) mutagenesis for increased production of extracellular lipase. Random mutagenesis was performed by incubating the spore suspension of fungus with EMS at a concentration of 5% (v/v) and 8% (v/v) for 30, 60 and 90 min, respectively. Control set was prepared by incubating the spore suspension with sterile distilled water. Control plate showed maximum number of fungal colonies whereas number of colonies was decreased as we increased exposure time of EMS from 30 to 90 min. The lipase activity of six mutagenic strains and wild strain was determined under submerged fermentation and solid state fermentation. Treated culture named as EMS5%-60min (obtained after 60 min exposure with 5% EMS) exhibited maximum activity (32.09 ± 1.84 IU/ml/min) in SmF as compared to wild strain (8.77 ± 3.52 IU/ml/min) and another treated strain named as EMS8%-90min (obtained after 90 min exposure with 8% EMS) exhibited maximum activity (7.99 ± 0.12 IU/g/min) in SSF as compared to wild strain (1.77 ± 0.71 IU/g/min). The activity of mutagenic strain i.e. EMS5%-60min was increased to 365.90% as compared to 100% activity of wild strain in SmF whereas activity of another mutagenic strain i.e. EMS8%-90min was increased to 451.41% as compared to 100% activity of wild strain in SSF.
Highlights
In 1940s the significance of microbial genetics was accomplished during penicillin production
Highest survival rate (100%) was observed on PDA plate inoculated with wild strain and survival rate was lowest (1.54%) on PDA plate inoculated with fungal spores after treatment with of 8% concentration of ethyl methane sulfonate (EMS) for 90 min (Table 2) [13]
Production of extracellular lipase was enhanced by strain improvement of local soil fungal isolate through random mutagenesis using two different concentrations (5%, 8% v/v) of EMS
Summary
In 1940s the significance of microbial genetics was accomplished during penicillin production. Mutagenesis came in existence by enlargement in productivity by thousand folds at industrial level and useful for fermentation industry. In commercial growth of microbial agitation the mutagenesis plays a fundamental role. Strain improvement through mutation and assortment is a much developed practice [1]. Wild strains obtained by primary and secondary screening produce low quantity of enzymes due to regulatory mechanism which controls the amount of enzyme synthesized; wild strains are not suitable for industrial enzyme production [2]. The productivity of wild strains can be increased by Horizon e-Publishing Group
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