Abstract
Co-culture techniques associating both dermal fibroblasts and epidermal keratinocytes have shown to have better clinical outcome than keratinocyte culture alone for the treatment of severe burns. Since fat grafting has been shown to improve scar remodelling, new techniques such as cell-therapy-assisted surgical reconstruction with isolated and expanded autologous adipose-derived stem cells (ASCs) would be of benefit to increase graft acceptation. Therefore, integrating ASCs into standardized procedures for cultured skin grafting could be of benefit for the patient if cell quality and quantity could be maintained. The purpose of this study was to evaluate ASC processing from adult tissue with simple isolation (without enzymatic steps), expansion (low density of 325-3,000 cells/cm 2 ) and storage conditions to assure methods to enhance the cellular resistance when transferred back to the patient. Co-culture with cell-banked skin progenitor cells (FE002-SK2) showed an increase of 40-50% ASCs yield at high passages alongside with a better preservation of morphology, proper adipogenic and osteogenic differentiation and efficient biocompatibility with 3D collagen scaffolds. ASCs can be considered as a valuable additional cell source to be delivered in biological bandages to the patient in a need of tissue reconstruction such as burn patients.
Highlights
Multipotent stem cells may be beneficial in tissue engineering applications for reconstructive surgery, for soft tissue enhancement and around implants for better integration [1,2] and potentially for burn patients [3,4,5,6].Until recently, the predominant source of stem cells considered for clinical applications was bone marrow-derived mesenchymal stem cells (BM-MSCs)
With standardized methods for adipose-derived stem cells (ASCs) culture using small quantities of tissue, these patient cells could be integrated into the co-cultured treatment for burns and perhaps provide a better overall treatment for skin and soft tissue reconstruction
More important is the actual recovery of cultured ASCs following freezing protocols to assure their growth stability and potential to stock patient cells until they are needed for therapeutic procedures
Summary
Multipotent stem cells may be beneficial in tissue engineering applications for reconstructive surgery, for soft tissue enhancement and around implants for better integration [1,2] and potentially for burn patients [3,4,5,6].Until recently, the predominant source of stem cells considered for clinical applications was bone marrow-derived mesenchymal stem cells (BM-MSCs). It is accepted that co-culture of both fibroblasts (dermal) and keratinocytes (epidermal) provides a better clinical outcome for burn applications [14]. With such patients, there is a lack of soft tissue and adipose tissue injection has been proposed to help in scar tissue remodelling [3]. With standardized methods for ASC culture using small quantities of tissue (that could be taken during initial debridement and biopsy for keratinocyte culture), these patient cells could be integrated into the co-cultured treatment for burns and perhaps provide a better overall treatment for skin and soft tissue reconstruction
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