Abstract
To enhance carotenoids production from <em>Rhodobacter sphaeroides</em>, Nitrosoguanidine (NTG) mutagenesis was employed to construct high-carotenoids <em>Rb. Sphaeroides</em> strain in the present study. Experimental results suggested that the NTG concentration and treat duration was 3 mg/mL and 15 min, respectively. One high-carotenoids <em>Rb. Sphaeroides</em> strain was finally obtained and the production of carotenoids was 2 times higher than that of the production from wild type <em>Rb. Sphaeroides</em>. The present study promotes to large scale production of natural carotenoids from <em>Rb. Sphaeroides</em>.
Highlights
Carotenoids are the most common, naturally occurring terpenoid pigments and responsible for yellow, orange and red color and synthesized in plants, algae and bacteria (Heider et al, 2014; Mata-Gómez et al, 2014)
Construction of high-carotenoids Rb. sphaeroides strain by NTG mutagenesis: A single colony was inoculated into a 50 mL-flask containing 40 mL of malate minimal media and grown under micro-aerobic conditions in dark at 30°C
The cell pellets Determination of total carotenoids: The absorbance were resuspended with 0.1 M PBS buffer and NTG solution was added to the resuspension to a final concentration of 3 mg/mL and treated for 40 min
Summary
Carotenoids are the most common, naturally occurring terpenoid pigments and responsible for yellow, orange and red color and synthesized in plants, algae and bacteria (Heider et al, 2014; Mata-Gómez et al, 2014). They possess a wide variety of biological functions, including anti-carcinogenic, immunemodulator and antioxidant activities (Liu et al, 2013; Maldonade et al, 2008). The present study will supply an insight into increasing carotenoids production by Rb. sphaerides and promote the industrial scale production of carotenods
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