Abstract
The effects of trehalose on the antagonistic activity of Pichia caribbica against Rhizopus decay and gray mold decay of strawberries and the possible mechanisms involved were investigated. The proteomic analysis and comparison of P. carribbica in response to trehalose was analyzed based on two-dimensional gel electrophoresis. The antagonistic activity of P. carribbica harvested from the culture media of NYDB amended with trehalose at 0.5% was improved greatly compared with that without trehalose. The PPO (Polyphenoloxidase) and POD (Peroxidase) activity of strawberries treated with P. carribbica cultured in the NYDB media amended with trehalose at 0.5% was higher than that of the strawberries treated with P. carribbica harvested from NYDB. The β-1, 3-glucanase activity of strawberries treated with P. carribbica cultured in the NYDB media amended with trehalose at 0.5% was also higher than that of the strawberries treated with P. carribbica harvested from NYDB and the control. Several differentially expressed proteins of P. carribbica in response to trehalose were identified in the cellular proteome, most of them were related to basic metabolism.
Highlights
The shelf-life of the strawberries is very short because of the postharvest fungal decay, which results in serious economic losses to strawberries
The disease incidence of Rhizopus decay of strawberries treated with P. carribbica which were harvested from the media of nutrient yeast dextrose broth (NYDB) amended with trehalose powder at 0.5% was 5%, which was significantly lower than that of the control strawberries (97.5%) and the strawberries treated with P. carribbica which were harvested from the media of NYDB (27.5%)
The antagonistic activity of P. carribbica was not enhanced through being incubated in the NYDB media amended with 0.2% or 0.8% trehalose, or NYTB media, compared with that incubated in NYDB without trehalose
Summary
The shelf-life of the strawberries is very short because of the postharvest fungal decay, which results in serious economic losses to strawberries. Synthetic fungicides are primarily used to control postharvest decay loss, a growing international concern over the often indiscriminate use of synthetic fungicides on food crops because of their possible harmful effects on human health [3]. Several biological control agents are effective in reducing postharvest decay caused by Rhizopus stolonifer and Botrytis cinerea on strawberry [5,6,7,8,9]. Like other non-fungicides means, currently all the biocontrol yeasts cannot reduce postharvest diseases as effectively as synthetic fungicides. For biological control to be accepted as an economically viable option, the efficacy of antagonistic yeasts in controlling postharvest disease must be enhanced [10]
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