Abstract
Colorectal cancer (CRC) is one of the most common cancers leading to comorbidities and mortalities globally. The rational of current study was to evaluate the combined epigallocatechin gallate and quercetin as a potent antitumor agent as commentary agent for therapeutic protocol. The present study investigated the effect of epigallocatechin Gallate (EGCG) (150mg) and quercetin (200mg) at different proportions on proliferation and induction of apoptosis in human colon cancer cells (HCT-116). Cell growth, colonogenic, Annexin V in addition cell cycle were detected in response to phytomolecules. Data obtained showed that, the colony formation was inhibited significantly in CRC starting from the lowest concentration tested of 10 µg/mL resulting in no colonies as visualized by a phase-contrast microscope. Data showed a significant elevation in the annexin V at 100 µg/mL EGCG(25.85%) and 150 µg/mL quercetin (48.35%). Moreover, cell cycle analysis showed that this combination caused cell cycle arrest at the G1 phase at concentration of 100 µg/mL (72.7%) and 150 µg/mL (75.25%). The combined effect of epigallocatechin Gallate and quercetin exert antiproliferative activity against CRC, it is promising in alternative conventional chemotherapeutic agent.
Highlights
Colorectal cancer (CRC) is one of the most common cancers leading to comorbidities and mortalities globally
Building upon the previous studies, we evaluated the effect of different concentrations of combined effect of quercetin and epigallocatechin Gallate (EGCG) on HCT116 CRC cells line
We evaluated the antiproliferative effect of EGCG and quercetin on HCT116 cells using WST-1 staining (Figure 1)
Summary
Colorectal cancer (CRC) is one of the most common cancers leading to comorbidities and mortalities globally. The present study investigated the effect of epigallocatechin Gallate (EGCG) (150mg) and quercetin (200mg) at different proportions on proliferation and induction of apoptosis in human colon cancer cells (HCT-116). The combined effect of epigallocatechin Gallate and quercetin exert antiproliferative activity against CRC, it is promising in alternative conventional chemotherapeutic agent. O presente estudo investigou o efeito de galato de epigalocatequina (EGCG) (150 mg) e quercetina (200 mg) em diferentes proporções na proliferação e indução de apoptose em células de câncer de cólon humano (HCT-116). Palavras-chave: câncer colorretal, galato de epigalocatequina, quercetina, apoptose. There is an urgent need to develop alternative or complementary treatment regimens to counteract the resistance to chemotherapy, concentration-limiting toxicities, off-target effects and relapse
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