Enhanced susceptibility to pulmonary infections after experimental stroke

Abstract

Event Abstract Back to Event Enhanced susceptibility to pulmonary infections after experimental stroke Claudia Dames1*, Levent Akyüz1, Odilo Engel1, Katarzyna Winek1, Hans-Dieter Volk1, Andreas Meisel1 and Christian Meisel1 1 Charité - Universitätsmedizin Berlin, Germany Bacterial infection is a relevant complication in patients with acute CNS injury and associated with worse neurological recovery and high mortality. Clinical and experimental evidence indicate that immunodepression is an important factor for the high incidence of pneumonia. Previous studies analysed stroke-induced suppression of immune responses in blood and lymphatic organs. Here, we analyse changes in cellularity and function of lung immune and epithelial cells by FACS and ex-vivo stimulation with different TLR-ligands and T-cell mitogens at different time points after cerebral ischemia induced by temporary occlusion of the middle cerebral artery in mice. In addition, we monitored pulmonary host responses in-vivo during the course of pneumococcal pneumonia by analyzing cellular changes and cytokine/chemokine levels in lungs of stroke mice and sham controls. Quantitative FACS analysis of cellularity at different time points after stroke shows decreased numbers of lymphocytes, dendritic cells and macrophages in lung. Functional analysis of immune cells indicates an impaired cytokine production in lung cells after ex-vivo TLR stimulation. In a model of induced pneumonia we analysed the in-vivo host response to Streptococcus pneumoniae. Preliminary data paradoxically suggest hyper-inflammatory responses in the early phase with an increase in lung cytokine levels in stroke mice.The correlation between lymphopenia and development of infectious complications is still incompletely understood. It seems that the mechanisms of immunodepression in lung are similar to those in the periphery. Defects in bacterial defences after stroke needs to be further investigated and may lead to new therapeutic strategies to prevent infections and improve outcome. Keywords: Stroke, Lung, Pneumonia, Streptococcus pneumoniae, mouse model Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Dames C, Akyüz L, Engel O, Winek K, Volk H, Meisel A and Meisel C (2013). Enhanced susceptibility to pulmonary infections after experimental stroke. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00583 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 May 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Claudia Dames, Charité - Universitätsmedizin Berlin, Berlin, Germany, claudia.dames@charite.de Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Claudia Dames Levent Akyüz Odilo Engel Katarzyna Winek Hans-Dieter Volk Andreas Meisel Christian Meisel Google Claudia Dames Levent Akyüz Odilo Engel Katarzyna Winek Hans-Dieter Volk Andreas Meisel Christian Meisel Google Scholar Claudia Dames Levent Akyüz Odilo Engel Katarzyna Winek Hans-Dieter Volk Andreas Meisel Christian Meisel PubMed Claudia Dames Levent Akyüz Odilo Engel Katarzyna Winek Hans-Dieter Volk Andreas Meisel Christian Meisel Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.