7-days of FREE Audio papers, translation & more with Prime
7-days of FREE Prime access
7-days of FREE Audio papers, translation & more with Prime
7-days of FREE Prime access
https://doi.org/10.3760/cma.j.issn.1001-9030.2015.05.046
Copy DOIPublication Date: May 8, 2015 |
Objective To investigate the role of canonical transient receptor potential 1 (TRPC1)in pneumonectomy(PE) plus subcutaneous injection of monocrotaline(MCT)- induced pulmonary artery hypertension(PH)in rats. Methods SD rats underwent unilateral lobectomy, and 1 week later, rats were intraperitoneally injected with 2% MCT(50 mg/kg).After 3 weeks, mean right ventricular pressure(m RVP) and right ventricular mass index(RVMI) were measured. The lung sections were stained by hematoxylin and eosin(HE)and observed under light microscope.Real - time reverse transcriptase- polymerase chain reaction(RT- qPCR) was performed to detect TRPC1 m RNA expression in rat pulmonary arteries(PAs).Store- operated calcium channel(SOCC) agonist cyclopiazonic acid(CPA)- induced PAs contraction was measured by vascular ring tension analysis and the intracellular Ca2+ concentration([Ca2+]i)of pulmonary artery smooth muscle cells (PASMCs) was monitored by Fluo3- AM assay.Meanwhile the effect of endothelin- 1(ET- 1)-induced vasocontration, and the relaxation of gadolinium(Gd3+) on ET- 1- induced PAs contraction was measured. Results In comparison to the control group, rats developed severe PAH, and right ventricular hypertrophy in PE+ MCT group.The expression of TRPC1 m RNA in control group was more than 3 folds of that in PE+ MCT group.CPA- induced vasoconstriction was increased from(16.6±1.5)% in control group to(126.6±15.4)% in PE+ MCT group(n= 8,P< 0.01), and Ca2+ influx in PASMCs evoked by CPA was enhanced from(268±76) nmol/L in control group to(1 832±867) nmol/L in PE+ MCT group(n= 8,P< 0.01).Gd3+- induced relaxation to Endothelin- 1 vasoconstriction was also potentiated from(36.9±3.7)% in control group to(70.1± 2.6)% in PE+ MCT group(n= 8,P< 0.05) Conclusion PE+ MCT- induced PH is associated with increased TRPC1 expression and TRPC/SOCC- induced store operated Ca2+ entry. Key words: Ca2+; Pneumonectomy; Monocrotaline
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.