Abstract
44 Introduction 45 Materials and Methods 47 Bacterial strains 47 Fermentation media 47 Fermentations 48 Recovery of propionicin PLG-1 48 Bacteriocin activity assay 49 Viable cell determination 50 Organic acid determinations 50 Effect of metal ions on bacteriocin production and stability 50 Results and Discussion 50 Standardized conditions for the well difiusion assay 50 Assessment of Lactobacillus delbrueckii ATCC 4797 as an indicator 52 organism Effect of Tween 80 on propionicin PLG-1 recovery 53 Modification of growth medium to improve bacteriocin production 55 Effect of metal ions on bacteriocin production and stability 56 Acknowledgments 57 References 58 PURIFICATION AND PARTL\L AMINO ACID SEQUENCE OF PROPIONICIN 65 PLG-1, A BACTERIOCIN PRODUCED BY PROPIONIBACTERIUM THOENII P127 Abstract 65 Introduction 6665 Introduction 66 Materials and Methods 67 Bacterial cultures and media 67 Production of propionicin PLG-1 68 Ammonium sulfate precipitation 68
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