Abstract
Due to growing health concerns, the urban population is utterly inclined towards a healthy lifestyle and incorporated nutritional food supplements to lower common health risks. The ω-3 and ω-6 PUFAs consumption is increasing, hence alternative commercial production is essentially developed. The microbial source is an emerging platform to overcome the global demand for omega PUFAs. Marine oleaginous protist Aurantiochytrium sp. found a potential source to produce substantial DHA and SFA. The objective of the present research was to enhance the PUFA yield by optimizing maximum tolerable glucose concentration with a suitable nitrogen ratio (10:1). The maximum lipid and DHA yield and content were determined 4.30, 1.34 g/L, and 62.4, 33.49 % of total biomass and lipid at 30 g/L glucose respectively, which is one of among highest reported, however relative PUFA was maximum 46.97 % (DHA) in total lipid at 10 g/L glucose. Remaining 42–53.6 % SFA could be used for biodiesel.
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