Abstract
Abstract Background Flavonoids of Hypericum perforatum are important secondary metabolites which have been widely utilized in medicine for a range of purposes. The use of methyl jasmonate (MeJA) elicitation for the enhancement of flavonoid production in cell suspension culture of H. perforatum would be an efficient alternative method for the flavonoid production. Results MeJA influenced the cells growth and flavonoid production. The optimal elicitation strategy was treatment of the cell cultures with 100 μmol/L MeJA on day 15, which resulted in the highest flavonoid production (280 mg/L) and 2.7 times of control cultures. The activities of catalase (CAT) were inhibited after MeJA treatment in the cell cultures, while the activities of phenylalanine ammonia lyase (PAL) increased, which led to the enhancement of flavonoid production. Conclusion MeJA elicitation is a useful method for the enhancement of flavonoid production in cell suspension culture of H. perforatum.
Highlights
Flavonoids of Hypericum perforatum are important secondary metabolites which have been widely utilized in medicine for a range of purposes
Callus induction and cell suspension culture establishment In the preliminary work, calyx, leaf, petal, and stem segments of H. perforatum were used as explants for callus induction
Some efforts have been made for the establishment of adventitious root cultures of H. perforatum for the production of flavonoids, phenolics, and xanthones [5,15,16,17]
Summary
Flavonoids of Hypericum perforatum are important secondary metabolites which have been widely utilized in medicine for a range of purposes. Commonly known as St. John's wort, is a perennial herb native to Europe and a traditional medicinal plant which has been utilized in Chinese folk medicine for a range of purposes [1,2]. Flavonoids, quercetin and its glycoside derivatives, comprise the major group of biologically active metabolites in H. perforatum and are important biochemical markers in authenticating the herbal plant materials [4,6]. The consumption of H. perforatum-derived products as pharmaceutical preparations or food additives has increased dramatically, and it is presently one of the most consumed medicinal plants in the world [4,7]. An alternative method for more efficient and controllable production of flavonoids from H. perforatum is urgently required
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