Abstract
Stimulating effects of conditioned medium (CM) addition and its combination with methyl jasmonate (MJA) elicitation on production of physiologically active taxuyunnanine C (TC) by cell cultures of Taxus chinensis were demonstrated in both a shake flask and a 1-l (working volume) concentric-tube airlift bioreactor. In control cell cultures, average cell growth rates were 0.117±0.003 per day in the flask and 0.086±0.001 per day in the airlift bioreactor. With addition of 50% (by volume) CM on day 0, an average growth rate of 0.153±0.006 per day in flask and 0.125±0.003 per day in bioreactor was obtained, and the maximum cell density (on day 11) was 15.0±0.1 g l −1 in flask and 15.5±0.8 g l −1 in bioreactor. TC production and productivity were 43.0±17.6 mg l −1 and 1.0±0.8 mg l −1 per day in bioreactor control cultures, and a much higher production of 78.9±20.6 mg l −1 and productivity of 4.5±1.2 mg l −1 per day were obtained in bioreactor cultures with CM addition. Combination of CM addition with MJA (at 100 μM) elicitation enhanced TC productivity in bioreactors by about 30% when compared with the cultures with only MJA elicitation, i.e., increased from 9.2±0.9 to 11.8±0.9 mg l −1 per day. The work is potentially beneficial to large-scale plant cell cultures for production of the bioactive secondary metabolite.
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