Abstract
Photo-oxidation is a problem for many fluorescent species, resulting in decreased photostability and a reduction in overall sample brightness. In order to increase the utility of fluorescence techniques, methods that slow the photo-decay of fluorophores must be pursued. Metal-enhanced fluorescence (MEF) is a technique1,2 that utilizes the plasmon resonance of nanoparticles to simultaneously enhance a given fluorophore's fluorescent emission and overall system-photostability. This enhanced photostability is possible due to a coupling in both the fluorophore's ground and excited state, with the emission of the coupled quanta originating from the nanoparticle itself. Despite the enhanced fluorophore photostability, the fluorophore is still susceptible to photo-oxidation. In this poster we demonstrate that the combination of solution-based antioxidants with MEF lends to more stable fluorescent solutions than is traditionally observed in fluorescence experiments.1. Aslan, K. and Geddes, C.D. (2010). “Metal-Enhanced Fluorescence: Progress towards a Unified Plasmon Fluorophore description.” In Geddes, C.D. (Ed.), Metal-Enhanced Fluorescence. New Jersey: John Wiley and Sons, pgs 1-24, ISBN: 978-0-470-22838-8.2. Dragan, A and Geddes, Chris D., (2012). Metal-Enhanced Fluorescence: The Role of Quantum Yield, Q0, in Enhanced Fluorescence. Applied Physics Letters, 100, 093115.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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