Enhanced peroxidase-like activity of porphyrin functionalized ceria nanorods for sensitive and selective colorimetric detection of glucose

Abstract

Ceria nanorods modified with 5,10,15,20-tetrakis(4-carboxyl phenyl)-porphyrin (H2TCPP) were prepared. These nanocomposites (H2TCPP–CeO2) exhibited the intrinsic peroxidase-like activity and could catalyze the oxidation of classical peroxidase substrate 3,3′,5,5′-tetramethylbiphenyl dihydrochloride (TMB·2HCl) in the presence of H2O2 to produce a typical color reaction from colorless to blue. Our results demonstrated that both the H2TCPP–CeO2 nanocomposites and CeO2 nanorods exhibited higher thermal durance than that of HRP. The affinity of The H2TCPP–CeO2 nanocomposites toward H2O2 and TMB is similar to that of HRP. Fluorescent results indicated that the catalytic mechanism of the H2TCPP–CeO2 nanocomposites were from the decomposition of H2O2 into hydroxyl radicals. Based on these studies, a simple, sensitive, and selective visual and colorimetric method using TMB as the substrate was designed to detect glucose when combined with glucose oxidase. The proposed colorimetric method can detect H2O2 at a low detection limit of 6.1×10−6M and a dynamic range of 10−5–10−4mol·L−1. This method can also detect glucose at a low detection limit of 3.3×10−5mol·L−1 and a dynamic range of 5.0×10−5–1.0×10−4mol·L−1.