Abstract
2599 Background: Interleukin-12 has been reported to induce type 1 helper T cells (Th1) that produce interleukin (IL)-2 and gamma-interferon (G-IFN), and recognized as one of the strongest immunotherapeutic agent. However, it was found that serum levels of type 2 cytokine such as IL-10 increased after systemic administration of IL-12. Methods: To study this paradoxical phenomenon, peripheral blood mononuclear cells (PBMC) were used collected from 7 healthy volunteer (HV) and 45 patients with gastric and colorectal cancer, and were used for this study. These PBMC were stimulated with IL-12 and the concentrations of E°-IFN, IL-6, IL-10 in the culture supernatant were measured as production of each cytokine after cultivation for 24 hours. Results: The production of IL-6 and IL-10 in patients were significantly higher than in HV. Those of IL-6 by patients with distant metastasis were higher than those by HV. Those of IL-10 were significantly increased in patients with nodal involvement and with distant metast...
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