Abstract
In this study, the role of extracellular polymeric substances (EPSs) in nitrogen removal and the microbial community structure of aerobic granular sludge (AGS) were analyzed under different dissolved oxygen (DO) conditions (6–7, 4–5, and 2–3 mg·L−1). The EPSs transported and retained nitrogen in the denitrification process, and the total inorganic nitrogen (TIN) in the EPSs decreased from 6.09 to 5.54 mg·g−1 MLSS when the DO concentration decreased from 6–7 to 2–3 mg·L−1. The microbial community showed different core denitrifying bacterial populations involved in nitrogen removal in the AGS system under different DO conditions, with more species when they were higher relative abundances of denitrifying bacteria participating in the nitrogen removal process in AGS under low DO conditions, including Hydrogenophilaceae, Thauera, Enterobacter, Xanthomonadaceae_unclassified, Comalmonadaceae_unclassified, Nitrosomonas and Paracoccus. This study provides a more comprehensive understanding of the DO effect on the TIN removal mechanism by AGS.
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