Enhanced microbial nitrate reduction using natural manganese oxide ore as an electron donor

Abstract

Nitrate contamination of groundwater is a global problem. Enhanced biological nitrate reduction by liquid organics combined with low-cost natural materials (as electron donors) can cost-effectively remove nitrate from groundwater. Dissolved Mn(II) as an electron donor has been thoroughly investigated to support microbial nitrate reduction. However, most Mn in soil and sediments is in solid form, and the ability of solid-phase natural manganese oxide ore (NMO) as electron donor and for supporting microbial nitrate reduction is unknown. Therefore, a microcosm experiment was conducted to bridge this gap in knowledge. The results demonstrated that microbial nitrate reduction (mainly converted to nitrite) was enhanced by NMO (rich in cryptomelane). The electrochemical and X-ray photoelectron spectroscopy analyses suggested that NMO may be oxidized by microbial metabolism. Illumina Miseq sequencing results indicated that Acidovorax spp. played a crucial role in NMO-supported nitrate reduction. Further Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analyses indicated that bacterial extracellular electron transfer may be one of the mechanisms for the microbial NMO oxidation. The results of our study highlight the potential importance of NMO in nitrate reduction in the natural environment and may pave the way for NMO-assisted technology for nitrate removal from groundwater with less usage of organic electron donors.