Enhanced hydraulic cleanability of biofilms developed under a low phosphorus concentration in reverse osmosis membrane systems

Chemical cleaning is vital for the optimal operation of membrane systems. Membrane chemical cleaning protocols are often developed in the laboratory flow cells (e.g., Membrane Fouling Simulator (MFS)) using synthetic feed water (nutrient excess) and short experimental time of typically days. However, full-scale Reverse Osmosis (RO) membranes are usually fed with nutrient limited feed water (due to extensive pre-treatment) and operated for a long-time of typically years. These operational differences lead to significant differences in the efficiency of chemical Cleaning-In-Place (CIP) carried out on laboratory-scale and on full-scale RO systems. Therefore, we investigated the suitability of lab-scale CIP results for full-scale applications. A lab-scale flow cell (i.e., MFSs) and two full-scale RO modules were analysed to compare CIP efficiency in terms of water flux recovery and biofouling properties (biomass content, Extracellular Polymeric Substances (EPS) composition and EPS adherence) under typical lab-scale and full-scale conditions. We observed a significant difference between the CIP efficiency in lab-scale (~50%) and full-scale (9–20%) RO membranes. Typical biomass analysis such as Total Organic Carbon (TOC) and Adenosine triphosphate (ATP) measurements did not indicate any correlation to the observed trend in the CIP efficiency in the lab-scale and full-scale RO membranes. However, the biofilms formed in the lab-scale contains different EPS than the biofilms in the full-scale RO modules. The biofilms in the lab-scale MFS have polysaccharide-rich EPS (Protein/Polysaccharide ratio = 0.5) as opposed to biofilm developed in full-scale modules which contain protein-rich EPS (Protein/Polysaccharide ratio = 2.2). Moreover, EPS analysis indicates the EPS extracted from full-scale biofilms have a higher affinity and rigidity to the membrane surface compared to EPS from lab-scale biofilm. Thus, we propose that CIP protocols should be optimized in long-term experiments using the realistic feed water.

Biofouling poses a significant challenge to reverse osmosis (RO) membrane systems, necessitating timely detection for effective control. This study evaluated the efficacy of flow cytometry (FCM) for early biofilm detection in comparison to conventional system performance indicators. Feed channel pressure drop and total cell concentration in the Membrane Fouling Simulator (MFS) flowcell cross-flow outlet water were monitored over time as early biofouling indicators. The results demonstrated the potential of increased bacterial cell concentration in cross-flow outlet water as a reliable indicator of biofouling development on the membrane. Water outlet monitoring enabled faster biofouling detection compared to feed channel pressure drop. Membrane autopsy confirmed biofilm presence prior to the pressure drop increase, highlighting the advantage of early detection in implementing corrective measures. Timely intervention reduces operational costs and energy consumption in membrane-based processes.

Biofouling is a problem that hinders sustainable membrane-based desalination and the stratification of bacterial populations over the biofilm’s height is suggested to compromise the efficiency of cleaning strategies. Some studies reported a base biofilm layer attached to the membrane that is harder to remove. Previous research suggested limiting the concentration of phosphorus in the feed water as a biofouling control strategy. However, the existence of bacterial communities growing under phosphorus-limiting conditions and communities remaining after cleaning is unknown. This study analyzes the bacterial communities developed in biofilms grown in membrane fouling simulators (MFSs) supplied with water with three dosed phosphorus conditions at a constant biodegradable carbon concentration. After biofilm development, biofilm was removed using forward flushing (an easy-to-implement and environmentally friendly method) by increasing the crossflow velocity for one hour. We demonstrate that small changes in phosphorus concentration in the feed water led to (i) different microbial compositions and (ii) different bacterial-cells-to-EPS ratios, while (iii) similar bacterial biofilm populations remained after forward flushing, suggesting a homogenous bacterial community composition along the biofilm height. This study represents an exciting advance towards greener desalination by applying non-expensive physical cleaning methods while manipulating feed water nutrient conditions to prolong membrane system performance and enhance membrane cleanability.

Enhanced biofilm solubilization by urea in reverse osmosis membrane systems

Biofouling control by phosphorus limitation strongly depends on the assimilable organic carbon concentration

Predicting the impact of feed spacer modification on biofouling by hydraulic characterization and biofouling studies in membrane fouling simulators

Periodic chemical cleaning with urea: disintegration of biofilms and reduction of key biofilm-forming bacteria from reverse osmosis membranes

Impact of organic nutrient load on biomass accumulation, feed channel pressure drop increase and permeate flux decline in membrane systems

Nutrient limitation has been proposed as a biofouling control strategy for membrane systems. However, the impact of permeation on biofilm development under phosphorus-limited and enriched conditions is poorly understood. This study analyzed biofilm development in membrane fouling simulators (MFSs) with and without permeation supplied with water varying dosed phosphorus concentrations (0 and 25 μg P·L−1). The MFSs operated under permeation conditions were run at a constant flux of 15.6 L·m2·h−1 for 4.7 days. Feed channel pressure drop, transmembrane pressure, and flux were used as performance indicators. Optical coherence tomography (OCT) images and biomass quantification were used to analyze the developed biofilms. The total phosphorus concentration that accumulated on the membrane and spacer was quantified by using microwave digestion and inductively coupled plasma atomic emission spectroscopy (ICP-OES). Results show that permeation impacts biofilm development depending on nutrient condition with a stronger impact at low P concentration (pressure drop increase: 282%; flux decline: 11%) compared to a higher P condition (pressure drop increase: 206%; flux decline: 2%). The biofilm that developed at 0 μg P·L−1 under permeation conditions resulted in a higher performance decline due to biofilm localization and spread in the MFS. A thicker biofilm developed on the membrane for biofilms grown at 0 μg P·L−1 under permeation conditions, causing a stronger effect on flux decline (11%) compared to non-permeation conditions (5%). The difference in the biofilm thickness on the membrane was attributed to a higher phosphorus concentration in the membrane biofilm under permeation conditions. Permeation has an impact on biofilm development and, therefore, should not be excluded in biofouling studies.

Analysis of reverse osmosis membrane performance during desalination of simulated brackish surface waters

In this study, the possibility of treatment of wastewater generated from underground hard coal mining excavation was experimentally investigated using Nano filtration (NF) and Reverse Osmosis (RO) membrane systems. Two-stage sequential treatment method was applied to perform the study. In the first stage, the raw wastewater was treated using NF membrane filtration system without any pre-processes. In the second stage, the effluents of NF membrane system were fed into RO membrane system for the treatment. To determine the treatment performance of the NF and RO membrane system, the operating pressures were fixed at 10, 20 and 30 bar (KN/m 2 ) for both systems during the experimental study. Turbidity, sodium, calcium, magnesium, manganese, iron, copper, aluminium, ammonium, sulphate and electrical conductivity were analysed in raw wastewater and permeate flow of NF and RO membrane systems to determine the treatment performances. Treatment performance of RO membrane system was observed to have highest yield of80% for all parameters examined at 20 bar operating pressure. Keywords: Polymeric membrane, wastewater treatment, coal excavation, water recycle and reuse

The ability to produce fresh potable water is an ever-growing challenge, especially with an increase in drought conditions worldwide. Due to its capacity to treat different types of water, reverse osmosis (RO) technology is an increasingly popular solution to the water shortage problem. The major restriction associated with the treatment of water by RO technology is the fouling of the RO membrane, in particular through biofouling. Membrane fouling is a multifaceted problem that causes an increase in operating pressure, frequent cleaning and limited membrane lifespan. The current paper summarizes the impact of biofouling of RO membranes used in seawater desalination plants. Following a brief introduction, the elements that contribute to biofouling are discussed: biofilm formation, role of extracellular polymeric substances (EPS), marine environment, developmental phases of biofouling. Following this, is a section on the implications of membrane biofouling especially permeate flux and salt rejection. The final section focuses on the new phenomenon of compression and hydraulic resistance of biofilms. Lastly, considerations on future research requirements on biofouling and its control in seawater reverse osmosis (SWRO) membrane systems are presented at the end of the article.

In this study, a hybrid process for leachate wastewater treatment including evaporation and reverse osmosis (RO) membrane or biological treatment systems was suggested. Experiments were performed on a real landfill leachate wastewater. The leachate was subjected to evaporation; as a result, a distillate was obtained containing less organic matter and less substantial amounts of other pollutants, as ammonium salts and total phenols were removed. Tests were carried out at different evaporation temperatures and times. The initial leachate pH was adjusted and optimized. For optimum conditions, each of chemical oxygen demand (COD), total phenol, and ammonium salt concentrations were reduced to 99.99%, 95.00%, and 83.00%, respectively. The distillate of the first stage of the proposed process was then exposed to RO membrane system, as a first study, under different transmembrane pressure of 20, 30, and 40 bar and at different pH values of 7, 8, and 9. As a second suggested treatment system, the distillate was subjected to a biological treatment process for 30 days as a retention time, pH = 6, and room temperature 25°C ± 1°C. At the end of the research study, a comparison was conducted between results obtained with RO membrane separation and biological treatment system as two distinct treatment systems proposed for leachate landfill wastewater treatment. Although both systems were effective for landfill leachate wastewater treatment, however, with the RO membrane separation system, COD removal efficiency reached 99.99%. In the other hand, with biological treatment process, COD elimination was as much as 90.00%. Certainly, evaporation and RO are not novel ways of landfill leachate treatment; however, few studies have attempted to use similar combined system for landfill leachate wastewater treatment and attained effective results of treated water. PRACTITIONER POINTS: A hybrid process of evaporation and RO membrane or biological treatment systems was suggested for leachate wastewater treatment. For optimum conditions, COD, total phenols, and ammonium salt reductions were achieved to 99.99%, 95%, and 83%, respectively, after the first evaporation stage. The distillate of the first stage of the proposed process was then exposed to RO membrane system and biological treatment system. Different transmembrane pressure and different pH values were optimized for RO.

Effect of water temperature on biofouling development in reverse osmosis membrane systems

Membranes are widely applied in water and waste water treatment as they provide an absolute barrier against the contaminants. Membranes are offered in wide pore size range and they are applied vastly due to their versatile and cost effective operation in dealing with wide range of streams. However, membranes, like any other filtration systems, suffer from fouling. Fouling layer, accumulation of rejected materials over time on membrane surface, is often called the main bottleneck of membrane processes. Fouling formation reduces water flux, increases energy consumption and leads to the early membrane replacement. To better control and mitigate fouling layer formation, better understanding of fouling mechanism and properties are required. Fouling properties can be categorized into hydraulic properties, mechanical properties, structural properties, chemical properties. These properties can be impacted by operational conditions, feed water quality and membrane properties. Moreover, these properties influence membrane performance parameters such as water flux, energy consumption and eventually the plant expenses. Therefore, the fouling properties, their inter-relations, their impacts on performance parameters should be further studied. We used novel modelling techniques and experimental measurements in laboratory and full-scale plants to study fouling properties and their impacts on membrane performance parameters. We also discussed the opportunities and challenges for future fouling study.Chapter 2. To evaluate the relation between structural, hydraulic and mechanical properties of fouling layer in the membrane systems, a novel method to extract these properties was developed to extracted fouling properties in a non-destructive and in-situ technique. The performance parameters of a dead-end UF system with integrated OCT imaging (in-situ) was coupled with a fully-coupled fluid-structural interaction (FSI) model. The dead-end UF was operated under a compression-relaxation cycle to evaluate how fouling properties changes under different applied pressure. Several mechanical models were evaluated to find the most suitable mechanical model to explain the fouling layer behaviour under compression-relaxation cycle in the dead-end UF. The results indicate that the hydraulic resistance of homogeneous biofilms under UF was much more affected by change in permeability than by the fouling layer thickness. Interestingly, we also found that even a poroelastic model (relatively simple model) can fairly good explain behaviour of the fouling layer in this study under different applied pressures. Compression of the fouling layer in UF systems can significantly increase hydraulic resistance of the membrane systems. In Chapter 2, a new technique was developed to extract fouling properties of the smooth surface biofilms. In Chapter 3 the new technique was further expanded to extract the mechanical properties of rough surface fouling layer under dead-end UF. We observed for the fouling layer which is fed with real surface water (i.e., river water), a dual-layer fouling structure with a thin and dense base layer and a thick and porous top layer could best explain the observed results. We also introduced a new fouling structure indicator, the fraction of exposed base layer, as a good indicator in the determination of water flux in UF systems.In Chapter 4 the chemical properties of fouling layer (e.g., composition) and their impacts on chemical cleaning efficiency in Reverse Osmosis (RO) systems were evaluated. Chemical cleaning protocols (often referred as CIP protocols) are usually developed under laboratory conditions (synthetic feed water, short-term experiments) and then are applied in the full-scale RO installations. This often leads to significant differences in CIP efficiency in the lab and full-scale installations. Thus, we compared the fouling layer properties and CIP efficiency of typical laboratory conditions RO and several full-scale RO plants. The results show that CIP efficiency in the full-scale RO plants are much lower than lab conditions RO. Later, we correlated such differences in CIP efficiency to their significantly different extracellular polymeric substance (EPS) properties. The EPS extracted from lab RO had different composition and adherence properties than the EPS extracted from full-scale RO. Therefore, we concluded that CIP protocols should not be developed under lab conditions. In the Chapter 5 we suggested a new method to better develop CIP protocols and study fouling properties with more industrial applications. We installed several new RO modules in the full-scale installation and they were operated for 30 days under identical conditions as the full-scale installation. Later, the fouling properties and CIP efficiency (in-situ measurement of permeability and pressure drop recovery) were compared between new RO modules (after 30 days of operation) and old RO modules (>2 years of operations). The new proposed fouling simulation method show promising results in both CIP efficiency results and fouling properties. Although fouling is inevitable part of filtration processes, its economic impacts on membrane systems is not well evaluated. In Chapter 6 cost of fouling in several RO and NF systems in The Netherlands has been calculated using plant performance data. All the cost factors contributing to cost of fouling such as CIP cost, energy cost, down cost were considered. We observed that for the RO plants, around a quarter of OPEX is caused by fouling, as oppose of around 10% for anoxic NF plants. The most important factor in the cost of fouling was considered the early membrane replacement cost, followed closely by additional energy cost. CIP costs have a minor contribution to the overall cost of fouling. Reuse of municipal wastewater effluent is part of solution to deal with water scarcity challenges. In Chapter 7 a fit-for-purpose approach to water reuse was proposed. We developed full techno-economic analysis on membrane-based Water reuse plant for municipal wastewater treatment effluent in the Netherlands. The impact of fouling and its properties and fouling cost have been integrated in all the membrane systems. A novel approach on design of water reuse plant has been offered to not only inherently reduce fouling impact but also increase plant robustness and water recovery. In chapter 8 a summarized and generalized conclusions of the previous chapters is presented. We also presents our suggestions and opportunities for the future membrane and fouling research.