Enhanced establishment of Colophospermum mopane (Kirk ex Benth.) seedlings for phytoremediation of Cu-Ni mine tailings

Abstract

This study was carried out to determine the effect of cow manure amendment and the method of planting on the growth, survival, and heavy metal accumulation of Colophospermum mopane seedling grown on Bamangwato Concessions Limited (BCL) mine tailings. Different planting strategies were employed where the mopane seedlings were planted with bare roots (devoid of potting soil) and without cow manure (T1, - CM - Soil); with the potting soil adhering to the roots but without cow manure (T2, - CM + Soil); with bare roots in the presence of cow manure (T3, + CM - Soil); and with potting soil intact together with cow manure (T4, + CM + Soil). Cow manure increased the pH of the mine tailings enhancing the survival and growth of the mopane seedlings. Seedlings grown under T1 conditions had a higher concentration of the heavy metals As, Cr, Cu, Mn, Ni, Pb, Zn, Sb, and Sr in their shoots compared to those grown under a T4 environment consisting of potting soil with cow manure. Cow manure decreased the availability of these heavy metals in mine tailings through the humic substance which adsorbed the heavy metal while the soil adhering to the roots diluted the concentration of heavy metals in the rhizosphere thus reducing the uptake and toxicity. Overall, the establishment of mopane seedlings in mine tailings could be enhanced by cow manure amendments and with soil adhering to its roots during transplanting.