Enhanced Efficiency of the Removal of Cytostatic Anthracycline Drugs Using Immobilized Mycelium of Bjerkandera adusta CCBAS 930.

Kamila Rybczyńska-Tkaczyk

doi:10.3390/molecules26226842

Kamila Rybczyńska-Tkaczyk

Open Access

https://doi.org/10.3390/molecules26226842

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Abstract

The aim of this study was to evaluate the bioremoval of anthracycline antibiotics (daunomycin-DNR, doxorubicin–DOX, and mitoxantrone-MTX) by immobilized mycelium of B. adusta CCBAS 930. The activity of oxidoreductases: versatile peroxidases (VP), superoxide dismutase (SOD), catalase (CAT), and glucose oxidase (GOX), and the levels of phenolic compounds (PhC) and free radicals (SOR) were determined during the biotransformation of anthracyclines by B. adusta strain CCBAS 930. Moreover, the phytotoxicity (Lepidium sativum L.), biotoxicity (MARA assay), and genotoxicity of anthracyclines were evaluated after biological treatment. After 120 h, more than 90% of anthracyclines were removed by the immobilized mycelium of B. adusta CCBAS 930. The effective biotransformation of anthracyclines was correlated with detoxification and reduced genotoxicity.

Highlights

IntroductionDaunomycin (daunorubicin, DNR), doxorubicin (DOX), and mitoxantrone (MTX) are used to treat acute myeloid and lymphocytic leukemias, chronic myelogenous leukemia, Kaposi’s sarcoma, and breast cancer [2,3]
Anthracyclines have been used for cancer treatment for over 50 years and are still included in the World Health Organization (WHO) model list of essential medicines [1].Daunomycin, doxorubicin (DOX), and mitoxantrone (MTX) are used to treat acute myeloid and lymphocytic leukemias, chronic myelogenous leukemia, Kaposi’s sarcoma, and breast cancer [2,3]
The immobilized cultures of B. adusta CCBAS 930 were characterized by a significant decrease in the color of the medium supplemented with the anthracycline antibiotics (90% after 120 h)

Summary

Introduction

Daunomycin (daunorubicin, DNR), doxorubicin (DOX), and mitoxantrone (MTX) are used to treat acute myeloid and lymphocytic leukemias, chronic myelogenous leukemia, Kaposi’s sarcoma, and breast cancer [2,3] The effectiveness of these cytostatic drugs is related to their presence in the environment. White rot fungi synthesize oxidoreductases, e.g., peroxidases, which facilitate the biodegradation of the lignocellulose complex of plant biomass Due to their wide range of substrates, fungal peroxidases can decompose compounds with an aromatic structure, e.g., pesticides, melanoidin, industrial dyes, post-industrial lignin, personal care products, and pharmaceuticals [8,14,16,17,18,19,20,21,22,23]

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