Enhanced Efficacy of Some Antibiotics in Presence of Silver Nanoparticles Against Multidrug Resistant Pseudomonas aeruginosa Recovered From Burn Wound Infections.

Maha A Khalil,Perihan S Ateya,Nanis G Allam,Sameh S Ali,Fatma I Sonbol,Gamal M El Maghraby

doi:10.3389/fmicb.2021.648560

Maha A Khalil, Perihan S Ateya + Show 4 more

Open Access

https://doi.org/10.3389/fmicb.2021.648560

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Abstract

Burn wound infections with multidrug-resistant (MDR) bacteria are shown in many countries as severe widespread health threats. Consequently, attention has been devoted to new nanoparticle-based materials in the field of antimicrobial chemotherapy for burn wound infections. This study aimed to evaluate both in vitro and in vivo efficacies of nanoparticle–antibiotic combinations as new classes of materials subjected against MDR Pseudomonas aeruginosa. Out of 40 Gram-negative isolates, 23 P. aeruginosa were recovered from patients with burn wound infections attending different hospitals in Tanta, Egypt. The susceptibility test revealed that 95.7% of P. aeruginosa isolates were MDR with a high incidence of resistance against carbenicillin. Antibacterial activities of silver nanoparticles (Ag-NPs) against the isolates examined showed various inhibition zone diameters ranging from 11 to 17 mm. Strong synergistic efficacy of neomycin was reported in combination with Ag-NPs against MDR P. aeruginosa P8 and P14 isolates. The in vivo effectiveness of various pharmaceutical formulations prepared from a combination of neomycin antibiotic with Ag-NPs in the treatment of induced bacterially infected mice burns showed that maximum healing activity along with faster wound contraction reported with the combination of neomycin-Ag-NPs in the spray formulation. Generally, data indicated that incorporating Ag-NPs in combination with certain antibiotics may be a new, promising application for wound treatments, especially burns infected with MDR P. aeruginosa.

Highlights

Burn wound infections are a significant cause of death in burn patients, those caused by MDR pathogens such as Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococci
Out of 94 bacterial isolates screened from 51 clinical swab samples taken from human burn wound infection, a total of 40 Gram-negative bacterial isolates were recovered (Figure 1)
P. aeruginosa isolates were selected for further study

Summary

Introduction

Burn wound infections are a significant cause of death in burn patients, those caused by MDR pathogens such as Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococci. These infections can result in healing failure and are used as a predictor of predicted mortality (Jain et al, 2009). There is an increasing demand for the development of novel alternatives to combat MDR pathogens (Khalil et al, 2015a, 2020) Under this scope, several metallic nanomaterials have superior antibacterial activity against MDR bacteria over traditional antibiotics. Their increased specific surface area enables interaction with bioorganic components on the viable cell surface of microorganisms, resulting in structural denaturation in the microbial cells (Alavi and Rai, 2019)

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