Abstract
The bioaugmentation role of microbes is often impeded by heavy metal (HM) ions in constructed wetlands (CWs). To explore the interaction between microbes and HM ions, two identical CWs: an MCW (with resistant microorganisms) and a CCW (as control) were used in this study. Experiments analyzed static adsorption performance in a synthetic HM solution. The removal performance of Cd2+ and Zn2+ was further investigated in both CWs. The removal efficiencies (REs) of 81.92–99.56% and 74.05–98.79% were achieved for Cd2+ and Zn2+ in the adsorption study, respectively. Significantly higher REs of Cd2+ (99.60%), and Zn2+ (94.41%) were achieved in the MCW. The microbial community analysis revealed that the dominant genera were Serratia and Pseudomonas in the MCW. The subcellular analysis further demonstrated that the HMs bioaccumulated mainly in the cytomembrane and cell wall. These results indicate that CW with resistant microorganisms inoculated was an effective strategy for treating HMs wastewater.
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