Abstract
Background: Aquaporins (AQPs), the family of water-selective channels, are localized in various organs and tissues, including the gastrointestinal (GI) tract. However, the roles of AQPs in the GI tract remain unclear. Materials and Methods: Male SD rats were subjected to subtotal colectomy (Group C, n = 22) or a sham operation (Group S, n = 16) and were sacri-ficed on postoperative days 7, 14, and 28. Total RNAs from the distal ileum and rectum were extracted. Quantitative RT-PCR was performed to measure AQP8 mRNA expression. For light-microscopy or immunohistochemistry, paraffin-embedded sections of 4 μm were prepared with H-E staining or anti-AQP8 antibody reaction. Mann-Whitney U-test was performed to compare the AQP8 distributions between the two groups, and the statistical significance was defined as p
Highlights
Water-selective channels have been identified as molecules located mainly on the plasma membrane of various cell types and increasing water permeability
Enhanced AQP8 mRNA expression in the subtotal colectomy model suggests that AQP8 plays an important role in maintaining the intestinal fluid balance
Previous studies have demonstrated the expression of several AQP types in the gastrointestinal (GI) tract, suggesting their participation in water absorption or secretion [1,4,5,9]. We have demonstrated both mRNA and protein expression of AQP1, AQP3, AQP4, and AQP8 in the rat GI tract [12,13]
Summary
Water-selective channels (aquaporins; AQPs) have been identified as molecules located mainly on the plasma membrane of various cell types and increasing water permeability. Previous studies have demonstrated the expression of several AQP types in the gastrointestinal (GI) tract, suggesting their participation in water absorption or secretion [1,4,5,9]. We have demonstrated both mRNA and protein expression of AQP1, AQP3, AQP4, and AQP8 in the rat GI tract [12,13]. Results: AQP8 mRNA expression was enhanced in both the ileum and rectum in Group C at day 7. Conclusion: Enhanced AQP8 mRNA expression in the subtotal colectomy model suggests that AQP8 plays an important role in maintaining the intestinal fluid balance
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