Abstract
Objective To explore the effect of colectomy on the expression of glucagon-like peptide-2(GLP-2) in serum and ileum and compare the changes of GLP-2 after different colectomy in rats. Methods Eighty male or female rats, aging 3-4 months old, were recruited in this study.The rats weight 180-250 g. The 80 rats were evenly and randomly distributed into 4 groups according to the surgical procedures they underwent: control group, in which the rats were not performed any procedures; sham surgery group, in which the rats underwent laparotomy; left hemicolectomy group, in which the rats were performed left hemicolectomy; and subtotal colectomy group, in which the rats were performed subtotal colectomy.According to execution time, each group had 4 subgroups, including 0 day group, 10 day group, 20 day group and 30 day group, and 5 rats were included in each subgroup.The whole blood was collected through cardiac puncture.Serum was collected and GLP-2 in serum was determined by enzyme-linked immunosorbent assay.The GLP-2 in ileum was studied by immunohistochemistry staining.The length of villus of ileum was also mea-sured by HE staining. Results The protein of GLP-2 was found in fibroblasts, epithelium and endocrine cells in ileum.Compared with control group and sham surgery group, the expressions of GLP-2 in serum and ileum increased significantly in the rats that underwent left hemicolectomy after surgery(all P 0.05). Conclusions The expressions of GLP-2 in serum and ileum are elevated after left hemicolectomy but not in subtotal colectomy, which may be related to the delayed intestinal adaption after colectomy. Key words: Glucagon-like peptide-2; Colectomy; Intestinal adaption
Published Version
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