Abstract
Acquisition of multidrug resistance and the spread of MDR among pathogenic microorganisms is a growing concern to world. Extended-spectrum ï¢-lactamases (ESBLs) strains of the Enterobacteriaceae; ESBL trait expressing Escherichia coli poses major threat and ESBL E. coli infections are increasingly reported from hospital settings as well as community settings with rising mortality and morbidity rates. The objective of this study was to study distribution of various types of ï¢-lactamases expressed within the clinical isolates of E. coli. In this work, clinical specimens: urine, stool, sputum, pus were collected over a period of three months from patients from rural area of Maharashatra State, India. Out of number of isolates, only thirty-six E. coli isolates were chosen for determining existence of ESBL, MBL, AmpC and Carbapenamase producing ability. Choice of isolates included resistance to at least of the three third generation cephalosprins used in this study. About 75% of E. coli strains were found to express ESBL trait, while remaining 25% expressed non-ESBL trait. About 28% isolates produced AmpC phenotype. Seven of 36 isolates (18%) indicated co-existence of ESBL and AmpC, while strain AKA43 was found to contain MBL and AmpC traits together. The prevalence of ESBL, MBL, AmpC and carbapenemase producing E. coli was studied. A few of these isolates were found to harbor more than one type of ï¢-lactamases. Key words: Extended spectrum ß-lactamases (ESBLs), ampCß-lactamases, metallo-ß-lactamases (MBLs), antibiotic resistance.
Highlights
The absence of new, effective anti-gram-negative antibiotics makes infection control the most important counter measure against multidrug-resistant gramnegative pathogens
Third world countries are considered as epicenter of this antimicrobial resistance primarily because the factors like overcrowding, poor nutrition and hygiene status and lack of infection control measures combined with antibiotic misuse/overuse has led to high endemic levels of resistant bacteria
Antibiotic susceptibility pattern for different β-lactams antibiotic was performed on E. coli isolates obtained from different clinical samples collected during May 2013- to July 2013
Summary
The absence of new, effective anti-gram-negative antibiotics makes infection control the most important counter measure against multidrug-resistant gramnegative pathogens. Extended-spectrum -lactamases (ESBLs) strains of Enterobacteriaceae have emerged as significant pathogens (Rawat, 2010). Third world countries are considered as epicenter of this antimicrobial resistance primarily because the factors like overcrowding, poor nutrition and hygiene status and lack of infection control measures combined with antibiotic misuse/overuse has led to high endemic levels of resistant bacteria. Continuous and improper use of third generation cephalosporins has induced mutations in β-lactamases and has led to the emergence of ESBL producing Enterobacteriacae members (Fair, 2014; Arora, 2005)
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