Abstract
The completion of genome sequences of pathogenic bacteria and the completion of human genome project has provided lot amount of data that can be utilized to design vaccines and drug targets. One of the recently adopting strategies for drug designing is based on comparative genomics approach, in which the subtraction dataset between the host and the pathogen genome provides information for a set of genes that are likely to be essential to the pathogen but absent in the host. This approach has been used to identify vaccine and drug targets of Pseudomonas aeruginosa and Helicobacter pylori . We have used the same approach to identify the vaccine and drug targets of Clostridium botulinum F strain . Our analysis has revealed that out of 3631 coding sequences of the pathogen, 446 represent essential genes that have no human homolog. We have further analyzed these 446 genes by the protein sequence database to list some 96 genes whose products are possibly exposed on the pathogen surface. This preliminary work reported here identifies a small subset of the Clostridium botulinum F strain proteome that might be investigated further for identifying potential drug and vaccine targets in this pathogen.
Highlights
The completion of human genome project and the completion of genome sequences of pathogenic bacteria have increased the momentum of field of drug discovery against threatening human pathogens
The work has been effectively complemented with the compilation of the Database of Essential Genes (DEG) for a number of pathogenic micro-organism [1,2,3,4,5].The whole approach is built on the assumption that the target should play an important role in the survival of the pathogen and it should not have any conserved homolog in the human host
Identification of non-human homologs in the essential genes of C.botulinum F strain with subsequent screening of the proteome to find the corresponding protein product are likely to lead to development of drugs that interact with the pathogen
Summary
The completion of human genome project and the completion of genome sequences of pathogenic bacteria have increased the momentum of field of drug discovery against threatening human pathogens. The target should be essential for growth and viability of the organism, should provide selectivity, and should yield a drug which is highly selective against pathogen with respect to human host. The work has been effectively complemented with the compilation of the Database of Essential Genes (DEG) for a number of pathogenic micro-organism [1,2,3,4,5].The whole approach is built on the assumption that the target should play an important role in the survival of the pathogen and it should not have any conserved homolog in the human host.
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