Abstract
This study evaluates the anti –Helicobacter pylori activity of Goldcrest honey at 10, 20, 50 and 75% v/v concentrations as well as its extracts (n-hexane, diethyl ether, chloroform and ethyl acetate) using the agar well diffusion technique. The minimum inhibitory concentrations (MIC50) of the two most active extracts were determined by the broth microdilution assay and MICs were read by enzyme-linked immunosorbent assay (ELISA) microtitre plate reader at 620 nm. The rate of kill of H. pylori strains by the most active extract was determined by viability studies over a period of 72 h. Data were analyzed by one-way analysis of variance (ANOVA) at 95% significance level. Goldcrest honey demonstrated anti-H. pylori activity, with the most potent activity at 75% concentration, with zone diameter in the range from 12.9 to 14.4 mm. Clarithromycin recorded a zone diameter of 18.0±7.4 mm not significantly different (P>0.05) from diethyl ether extract, with a zone diameter of 19.9±10.1 mm. MIC50 values of n-hexane and diethyl ether extracts were in the range of 0.039 to 10% and 0.078 to 10% (v/v), respectively. The bactericidal effect of n-hexane extract was highest at 4 × MIC concentration, within 30 to 72 h during which 100% of bacterial cells were killed. Goldcrest honey and its solvent extracts may contain potential lead molecules with anti-H. pylori activity. Further studies are therefore needed to determine their phytochemical constituents and activity. Key words: Goldcrest honey, antibacterial activity, minimum inhibitory concentrations (MIC), time kill assay, Helicobacter pylori.
Highlights
Helicobacter pylori (H. pylori) has probably been part of the human gastric biota since time immemorial (Blaser, 1997)
The minimum inhibitory concentrations (MIC50) of the two most active extracts were determined by the broth microdilution assay and MICs were read by enzyme-linked immunosorbent assay (ELISA) microtitre plate reader at 620 nm
At various concentrations (10, 20, 50 and 75% v/v), the honey demonstrated antibacterial activity against H. pylori strains with zone diameters that ranged from 12.9 to 14.4 mm, with the most potent activity (20/30, 66.7%) at 75%v/v concentration (Table 1)
Summary
Helicobacter pylori (H. pylori) has probably been part of the human gastric biota since time immemorial (Blaser, 1997) It is a human pathogen of major public health concern since it is directly associated with many diseases of the upper gastrointestinal tract including acute and chronic gastritis, non-ulcer dyspepsia, peptic ulcer disease (gastric and duodenal ulcers) and gastric cancers (McColl, 2010), which are the major causes of death worldwide. Eradication of this pathogen is a major step in the therapeutic management of the aforementioned diseases (O'Connor et al, 2011). Honey is among the attractive sources that has received attention as an alternative treatment for H. pylori infections
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
