Abstract
English
Highlights
Hematopoietic stem cells (HSCs) can self-renew and produce progenitors that are committed to differentiate in to a wide range of blood cell types, including erythrocytes, leukocytes, lymphocytes, and platelets
As part of our approach, we examined the kinetics of leukemic cell growth in the presence of the anti-cancer drug, cytarabine (Ara-C), in our 3D culture system compared with 2D culture models
Cytarabine was purchased from Sigma-Aldrich Co., dissolved in pyrogen-free saline to produce a concentration of 1 mg/mL, diluted with Iscove’s modified Dulbecco’s medium (IMDM) for use
Summary
Hematopoietic stem cells (HSCs) can self-renew and produce progenitors that are committed to differentiate in to a wide range of blood cell types, including erythrocytes, leukocytes, lymphocytes, and platelets. Earlier studies demonstrated that the proliferation and differentiation of HSCs are regulated by the bone marrow microenvironment [1,2,3]. Stromal cells, which are distinct from hematopoietic cells, are an essential component of this microenvironment, and are necessary for the long-term maintenance of HSCs in vitro [4, 5]. Stromal cells within the bone marrow microenvironment influence the proliferation of leukemic cells, as well as the normal hematopoietic cells from which they were derived. The ability of stromal cells to support the proliferation (and differentiation) of HSCs or leukemic cells and to maintain their self-renewal potential has generally been investigated in long-term, two-dimensional (2D) bone marrow culture systems, which are structurally fundamentally different from the Corresponding author: Tomonori HARADA, M.D., Department of Functional Morphology, Nihon University School of Medicine, 30-1 Oyaguchikamicho, Itabashi-ku, Tokyo 173-8610, Japan. Tel.: 81-3-39728111 ext. 2222; Fax: 81-3-3973-8832; Email: harada.tomonori@nihon-u. ac.jp
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