Abstract
In this study, the genetic diversity of 6 oak species known as Quercus coccifera L., Q. robur L., Q. infectoria Oliver, Q. cerris L., Q. ithaburensis subsp. macrolepsis (Kotschy) Hedge and Yalt. and Q. trojana P.B. Webb in 18 populations was screened using the randomly amplified polymorphic DNA method (RAPD). 10 RAPD primers giving the best results produced 262 total loci. The highest and lowest band sizes were between 125 and 1800 bp, respectively. The binary RAPD data was computed using the Statistica version 8.0 and Popgene 32, genetic data analysis software program. The principal component analysis and cluster analysis displayed the seperation of populations based on genetic distances. The genetic similarity and distance matrix using Popgene 32 based on Nei (1972) revealed the genetic relations between studied populations. As a result of this study, it may be expressed that genetic relationships are more similar in the species belonging to same section and especially the relationships between Quercus cerris and Quercus trojana in the section Cerris attracts quite attention. Key words: Cerris, genetic relationships, Quercus, randomly amplified polymorphic DNA method (RAPD).
Highlights
Quercus L. (Oak) is a member of family Fagaceae containing very important woody plants (Jawarneh et al, 2013; Alfonso-Corrado et al, 2014)
Despite the shortcomings like problems related to reproducibility in amplification of randomly amplified polymorphic DNA method (RAPD) markers, inadequacy to distinguish between heterozygotes and homozygotes, the RAPD method having advantages such as the high level of polymorphism and applicability was prefered as molecular technique to evaluate 6 oak species
The results show that the method could reveal the genetic relationship among six species of oaks and distinguish them
Summary
Quercus L. (Oak) is a member of family Fagaceae containing very important woody plants (Jawarneh et al, 2013; Alfonso-Corrado et al, 2014). (Oak) is a member of family Fagaceae containing very important woody plants (Jawarneh et al, 2013; Alfonso-Corrado et al, 2014). The genus has a natural distribution in the northern hemisiphere in the world with high diversity (Govaerts and Frodin, 1998; Jawarneh et al, 2013; Laakılı et al, 2016). Turkey with 18 species of oaks is an important region with high species diversity (Yaltırık, 1984; Borazan and Babaç, 2003). Before the classification of Hedge and Yaltırık, many intraspecific taxa were classified as species and species concept for Quercus taxa was quite narrow (Borazan and Babaç, 2003). There are still unresolved nomenclatural and typification problems today
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