Abstract
Effect of herbal medicine on cell migration is one of the therapeutic points. The objective of this study was to compare the Scratch assay with the Boyden chamber assay for evaluating cell migration with Aloe resin as the chemoattractant agent. Human gingival fibroblasts cells (2.5×105 cells/ml) were exposed to 0.02% (w/v) of Aloe resin in 6-well culture plates for the Scratch assay or in 24-transwell-culture plates for the Boyden chamber assay. Cell migration into the scratch area and at the bottom of a transwell insert after 24 and 16 h exposure to Aloe resin were stained with Toluidine blue O and Giemsa stain, respectively. Images of the migrated cells in each assay were obtained using a microscope and counted. The percentage of cell migration was 29.94±3.5 and 32.4±2.5 in the Scratch assay and Boyden chamber assay, respectively. There was no significant difference in cell migration between the two assays (p>0.05). The ICC values of both assays in 5 experiments were 0.767, 0.792, 0.816, 0.758, and 0.778. The strength of agreement for both assays was good. The Boyden chamber assay can be used for cell migration evaluation and generated results similar to those of the Scratch assay. Key words: Aloe resin, intraclass correlation coefficient, wound healing assay.  
Highlights
Cell migration is the process of individual cells or cell clusters moving from one location to another
Cell migration occurs either pathologically or physiologically in humans based on the outcome, including wound healing, cell growth, cell differentiation, and cell proliferation (Martin, 1997)
The cells are exposed to the potential therapeutic agent or chemoattractant agent for wound healing
Summary
Cell migration is the process of individual cells or cell clusters moving from one location to another. Cell migration occurs either pathologically or physiologically in humans based on the outcome, including wound healing, cell growth, cell differentiation, and cell proliferation (Martin, 1997). Cell migration in wound healing occurs cooperatively between several cell types, including keratinocytes and fibroblasts, which provide growth factors (Martin and Leibovich, 2005). There are multiple techniques for evaluating cell migration in wound healing, such as the Scratch assay, Boyden chamber assay, and microfluidic chamber assay. The Scratch assay was the first assay used for evaluating cell migration (Liang et al, 2007). A wound gap is created by scratching a straight line on a confluent cell monolayer cultured in a petri dish or tissue culture plate with a pipette tip. Images of the cell migration across the gap are obtained using a microscope over a 24 h incubation period and evaluated
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