Abstract
This work made use of the strong interaction between biotin and streptavidin to connect designed DNA origami structures. The caDNAno software was used to design a 6 layer 3D origami cross-like structure. Selected DNA strands at the engineered attachment sites on the DNA origami structure were biotinylated. After folding of the origami structures, the biotinylated strands stick out of the attachment sites. Purified samples of origami structures were then mixed with streptavidin and the mixture purified. After characterization, we see that attachment only occurs at the biotinylated sites. Agarose gel electrophoresis, UV-vis spectroscopy and TEM were used to characterize the structure. Key words: DNA origami, interaction, biotin-streptavidin, nanomaterials, TEM.
Highlights
The specific binding of bases is exploited to selfassemble DNA which gives a large amount of control over nanoscale devices assembly. Seeman (1982, 2003) laid down the theoretical model that allowed the use of DNA as a building material for the construction of devices at the nanoscale
We demonstrate the use of this interaction to connect two or more DNA origami structures
Many of the research associating DNA origami with the biotin-streptavidin interaction has focused on decorating the origami template with other functional materials (Eskelinen et al, 2011; Lyonnais et al, 2008)
Summary
The specific binding of bases is exploited to selfassemble DNA which gives a large amount of control over nanoscale devices assembly. Seeman (1982, 2003) laid down the theoretical model that allowed the use of DNA as a building material for the construction of devices at the nanoscale. This work made use of the strong interaction between biotin and streptavidin to connect designed DNA origami structures. The caDNAno software was used to design a 6 layer 3D origami cross-like structure.
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