Abstract
The versatility of the DNA origami approach of organizing nanoparticles at the nanometer scale, together with thiol chemistry have been used. These approaches were used to design DNA origami structures and to functionalize them with gold nanoparticles after designing attachment sites on the DNA origami structures. In all two structures were designed – a cross-like structure and a nanotube but only the nanotube structure was used to form the gold nanoparticle helices. Finally, use was made of the specific affinity interaction between biotin and streptavidin to connect the DNA origami templated AuNP helices to the cross-like structure. Agarose gel electrophoresis, UV-vis spectroscopy and TEM were used to characterize the structure.
Highlights
On the nanometer scale matter often behaves differently with respect to the bulk properties of the same material
The solution was mixed with purified 20 μl of the nanoheliced DNA origami nanotube solution and the mixture incubated for two days at 4 0C
Use was made of the strong specific affinity interaction between biotin and streptavidin to connect the DNA origami templated structures
Summary
On the nanometer scale matter often behaves differently with respect to the bulk properties of the same material. This has initiated a wealth of research work to understand, characterize, describe and predict what happens at the nanoscale level (Moriarty, 2001; Rosei, 2004; Ratner & Ratner, 2003). There are many established synthetic routes to the production of nanoparticles of controlled morphology and composition, the programmed spatial arrangement of nanoparticles remains a challenge. Self-assembly provides an attractive alternative if appropriate methods can be developed which will allow control over size and geometry of designed objects. There are many strategies and approaches available for bioconjugation of nanoparticles, including attachment to biotin-avidin (Bruchez, Moronne, Gin, Weiss, & Alivisatos, 1998; Connolly & Fitzmaurice, 1999), antigen-antibodies (Shenton, Davis, & Mann, 1999), peptides (Whaley, English, Hu, Barbara, & Belcher, 2000; Hermanson, 1996), proteins (Hermanson, 1996), DNA (Storhoff & Mirkin, 1999), etc
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