Abstract
Zerumbone (ZER) is a sesquiterpene derived from Zingiber zerumbet smith, family Zingiberaceae. It has been shown to possess anti-cancer and apoptosis-inducing properties against various human tumour cells as well as in vivo against a number of induced malignancies in mice. In this study a simple, specific and accurate high performance liquid chromatographic method for determination of ZER in micro-volumes human plasma (| 1.5 ml) was developed and validated. ZER and its analogue α-Humulene as internal standard were easy recovered by simple one step plasma protein precipitation using acetonitrile and separated in isocratic mobile phase, on reverse phase-C18 column. The effluent was monitored by Photodiode Array (PDA) detector and at a flow rate of 1.0 ml/min. The linearity of proposed method was 2 – 15 µg/ml. The intra-day and inter-day coefficient of variation and percent error values of the method were less than 15% and mean recovery was more than 90% for both ZER and α-Humulene. This method was found to be precise, specific, accurate and robust for detection and analysis of ZER in human plasma. Key words: ZER, humulene, HPLC, human plasma.
Highlights
Zingiber zerumbet (L.) smith, known as lempoyang, is a member of the family Zingiberaceae, used in the traditional medicine as a cure for swelling, sores, loss of appetite and worm infestation in children (Somchit and Nur-Shakirah, 2003)
In some Southeast Asian countries, the rhizomes of the plant are employed in traditional medicine as anti-inflammation, while the young shoots and inflorescence are used as condiments (Murakami et al, 2002)
The chromatographic conditions and sample preparation for the proposed analytical procedure were optimized to be suitable for preclinical pharmacokinetic studies
Summary
Zingiber zerumbet (L.) smith, known as lempoyang, is a member of the family Zingiberaceae, used in the traditional medicine as a cure for swelling, sores, loss of appetite and worm infestation in children (Somchit and Nur-Shakirah, 2003). In some Southeast Asian countries, the rhizomes of the plant are employed in traditional medicine as anti-inflammation, while the young shoots and inflorescence are used as condiments (Murakami et al, 2002) This plant has been shown to have anti-tumor (Sakinah et al, 2007), anti-inflammatory (Murakami et al, 2002) and cyclooxygenase-2 suppressant properties (Tanaka et al, 2001). Zerumbone (ZER) is a bioactive crystalline monocyclic sesquiterpene derived from zingiber zerumbet rhizomes It exhibits variety of interesting reactions, such as region-and regeoselective conjugates additions, transannular ring contraction, cyclization and several regeospecific reactions which cleave the 11-membered ring (Kitayama et al, 1999, 2001; Ohe et al, 2000). This compound showed a potential candidate for the development of anticancer treatment of natural origin
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