Abstract
This study was done to determine minimum inhibitory concentration (MIC) of the aqueous extracts of garlic (Allium sativum), mint (Menthe spp.) and onion (Allium cepa) in in vitro conditions against the Escherichia coli isolated from broiler chickens. E. coli was isolated from the infected tissues of the chickens which were suspected of Colibacillus infection. In this study, distilled water, phenol phenicol and floxacin antibiotics were used as control. E. coli was sensitive to antibiotics, but distilled water had no inhibitory effect on the activity of E. coli. In this experiment, each of the aqueous extracts was prepared by using distilled water in 6 concentrations: 0.5, 1, 2.5, 5, 10 and 20%, and was poured into the cavities innutrient agar medium, and then the plates were kept in incubator at 37°C for 24 h. The results indicated that MIC of the garlic aqueous extract was 5%, but E. coli was resistant to the aqueous extracts of onion and mint. Key words: Herbal extracts, garlic, onion, mint, Escherichia coli, broiler chickens, minimum inhibitory concentration, antibacterial effects.
Highlights
Pharmaceutical plants have been commonly used by humans long ago and the consumption rate has changed based on the occasion and the requirement of the time
The results indicated that minimum inhibitory concentration (MIC) of the garlic aqueous extract was 5%, but E. coli was resistant to the aqueous extracts of onion and mint
The aim of this study is to evaluate the antibacterial effects of 3 herbal extracts- garlic (Allium sativum) mint (Menthe spp.) and onion (Allium cepa) on E. coli in vitro conditions and to determine the minimum inhibitory concentration (MIC) of these herbal extracts
Summary
Pharmaceutical plants have been commonly used by humans long ago and the consumption rate has changed based on the occasion and the requirement of the time. There have been several attempts to develop these plants and the derivatives because the ever-increasing usage of the chemical drugs has led to serious problems and created resistant types of insensitive micro-organisms, while herbal drugs bring about fewer side effects because the biological balance. It is a facultative anaerobic bacterium that grows on certain mediums especially eosin methylene blue agar (EMBA) with metallic sheen colonies. This organism grows at the range of 10 to 46°C but optimal temperature is 37°C. This organism, in different conditions, can act as a pathogenic bacterium, especially in secondary infections
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