Abstract
The immune protective effects of pseudorabies virus (PRV) vaccines in the market have gradually reduced and they have failed to provide complete protection against the new PRV variant. In this study, a triple gene-deleted live PRV strain–rZDΔTK-gE-gI was successfully constructed by simultaneously knocking out the three major virulence genes (gE/gI and TK) with CRISPR/Cas9 and Cre/LoxP gene editing system and low melting point agarose purification method. After challenge with the virulent PRV variant, all the 3-week-old piglets vaccinated with the rZDΔTK-gE-gI PRV vaccine candidate survived without any clinical symptoms, whereas all the unvaccinated piglets exhibited PRV respiratory and neurological signs with 100% mortality rate within 7 days post infection. High levels of anti-gB antibodies were induced in the vaccinated piglets after vaccination with the rZDΔTK-gE-gI vaccine candidate, which elicited a better immune protective effect than the classical strain Bartha-K61. Therefore, the triple gene-deleted live PRV vaccine candidate is expected to control the current outbreak of pseudorabies caused by the PRV variants.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
