Abstract
Thirty (30) simple sequence repeat (SSR) primer pairs chosen randomly from the SSR primer collection were used to detect polymorphism in 17 sugarcane accessions. A total of 62 DNA fragments were generated by the 30 primers with an average of about 2.14 bands per primer. Bands that a primer yielded in the study ranged from 1 to 4. The genetic distances for SSR data using 17 sugarcane accessions, was constructed based on Nei (1978) and relationships between accessions were portrayed graphically in the form of a dendrogram. The value of genetic similarity ranging from 62.90 to 90.30% was observed among the 17 sugarcane accessions. The highest genetic similarity of 90.03% was seen among genotypes S-2003-US-118 and S-2003-US-312. From the present study, it may be concluded that SSRs markers are best tool for investigation of genetic diversity in sugarcane. Key words: Simple sequence repeat (SSR), polymorphism, genetic diversity.
Highlights
Sugarcane (Saccharum spp. hybrids) is a genetically complex crop of major economic importance in tropical and sub-tropical countries (Khan et al, 2004)
The main reasons which make microsatellites an especially attractive tool for a number of applications are: their high levels of allelic variation and their co-dominant character, which means that they deliver more information per unit assay than any other marker systems, reducing costs; microsatellites are assayed using polymerase chain reaction (PCR), so only small amounts of tissue are required
The PCR product was observed by running on agarose gel to study polymorphism, most of the primers were polymorphic except five primers which were monomorphic and produced only one fragment per primer (Figure 1)
Summary
Sugarcane (Saccharum spp. hybrids) is a genetically complex crop of major economic importance in tropical and sub-tropical countries (Khan et al, 2004). It is mainly used for sugar production but recently gained increased attention because of its employment generation potential and recent emphasis on production of bio-fuels. The importance of sugarcane has increased in recent years because cane is an important industrial raw material for sugar and allied industries producing alcohol, acetic acid, butanol, paper, plywood, industrial enzymes and animal feed (Arencibia, 1998). Considering the current needs of cane industry it is imperative to breed high sugar producing varieties that have other desired agronomic traits. Sugar recovery can be increased from current average of 8.32
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