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Abstract
The aroma or fragrance of Basmati rice is associated with the presence and content of the chemical compound, 2-acetyl-1-pyrroline and the trait is monogenic recessive. Several polymerase chain reaction (PCR)-based co-dominant markers based on RG28 locus were developed, which can differentiate between fragrant and non-fragrant rice cultivars. For molecular and biochemical analysis of aroma, a mapping population comprising 208 recombinant inbred lines (RILs) derived from a diverse cross between CSR10 and Taraori Basmati through Single seed descent (SSD) method was used. RILs are among the best mapping populations, which provide a novel material for linkage mapping of genes/QTLs marker for various traits. Biochemical analysis of aroma was performed with the 1.7% KOH solution and molecular analysis of aroma was carried out with microsatellite markers present on chromosome 8 (BAD2, BADEX7-5, SCUSSR1) to determine the extent of association between trait, marker and chromosome 8. Among these markers, BAD2 amplified aroma specific alleles having 256 bp in 72 lines, BADEX7-5 with 95 bp in 74 lines and SCUSSR1 with 129 bp in 79 lines. Mantel test of significance detected by biochemical analysis of RILs (with 1.7% KOH) and molecular marker study revealed high degree (>90%) of association of aroma with the above mentioned markers, respectively. Some of the F10 lines amplified the heterozygous alleles for two sets of specific markers (BAD2 and SCUSSR-1) but did not show the presence of aroma as analyzed by chemical test. Aromatic and nonaromatic lines were almost common in three markers, indicating association of markers with the trait and chromosome 8. The results reveal that these markers could be used for marker assisted selection and RIL population for mapping of aroma QTLs/genes.
Highlights
Aromatic rice varieties constitute a small but special group of rice and have gained greater importance with the worldwide increase in the demand for fine quality rice (Sun et al, 2008)
Several polymerase chain reaction (PCR)-based co-dominant markers based on RG28 locus were developed, which can differentiate between fragrant and non-fragrant rice cultivars (Garland et al, 2000; Cordeiro et al, 2002; Sakthivel et al, 2009)
A F 10 recombinant inbred lines (RILs) mapping population was developed by single seed descent method from a cross of diverse parents CSR10 x Taraori basmati for purpose of linkage mapping
Summary
Aromatic rice varieties constitute a small but special group of rice and have gained greater importance with the worldwide increase in the demand for fine quality rice (Sun et al, 2008). In any molecular breeding program, development of suitable permanent mapping population for linkage studies is a prerequisite. Recombinant inbred lines (RILs) among various mapping populations provide a noble material for linkage of marker and trait. These are one of the most widely used populations for gene/QTL mapping (Burr and Burr, 1991). Linkage maps obtained are permanent and suitable for genetic studies Keeping this in view, a F 10 RIL mapping population was developed by single seed descent method from a cross of diverse parents CSR10 (non-aromatic, high yielding) x Taraori basmati (aromatic, poor yielder) for purpose of linkage mapping. The genotyping and phenotyping of this population was done for aroma using molecular markers and biochemical test
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