Abstract
Microalgae are a promising alternative source to produce biofuels and bio-products, as they consume carbon dioxide to grow, multiply quickly, and can be cultivated in domestic and industrial wastewater. The efficiency of microalgae-based biodiesel industry depends on the selection of appropriate strains with best lipid content yields and adequate fatty acids composition. The aim of this study was to select a potential microalga strain for lipid production by bioprospecting and screening lipid productivity and fatty acid compositions of some local microalgae isolated from different water bodies located in the region of Agadir, Morocco and cultivated in homemade photobioreactors. For this purpose, four freshwater microalgae Parachlorella kessleri, Cyclotella species, Chlorella species and Closterium species were isolated and cultivated. Lipid extraction was carried out using Soxhlet and fatty acids profiling was performed using Gas Chromatography. The highest lipid content was obtained with a diatom microalga Cyclotella spp. (33 wt%) after 15 days of residence time. The investigated strains produced high amount of oleic acid (41-54%) followed by linoleic (18-33%), palmitic (13-23%) and stearic acid (4-12%), respectively. Variations in lipid production and fatty acids composition as responses to changes in residence times form another important factor to feedstock selection for biofuel production. Key words: Biofuel, biomass, culture time, fatty acid, freshwater microalgae, lipid.
Highlights
Biodiesel, as derived from vegetable oils or animal fats, is an alternative diesel fuel that is becoming accepted in a steadily growing number of countries around the world (Ganesan et al, 2020).there is a huge interest in using microalgae to produce oil for biodiesel because of their potential for high productivity in shorter time and because they hold the potential to provide a scalable renewable feedstock, without harming the food supply (Chisti, 2007)
In order to determine the best residence time of the culture inside the PBR for biomass and lipid production, microalga P. kessleri selected as a control strain and three native microalgae strains were isolated and cultivated in 25 L PBR under five different residence times
This paper examines the effect of residence time of microalgae in PBR on lipid production and fatty acid composition, and to the best of our knowledge, this factor has never been reported on any study
Summary
As derived from vegetable oils or animal fats, is an alternative diesel fuel that is becoming accepted in a steadily growing number of countries around the world (Ganesan et al, 2020).there is a huge interest in using microalgae to produce oil for biodiesel because of their potential for high productivity in shorter time and because they hold the potential to provide a scalable renewable feedstock, without harming the food supply (Chisti, 2007). As derived from vegetable oils or animal fats, is an alternative diesel fuel that is becoming accepted in a steadily growing number of countries around the world (Ganesan et al, 2020). An increasing number of researches are in progress to enhance microalgae lipid production systems but there is barely any research dealing with the quality of this feedstock (Stansell et al, 2012). Two biodiesel standards that have served in the development of other standards around the world are the standards ASTM D6751 in the United States and EN 14214 in Europe. The development of those standards has accompanied the increasing interest in production and use of biodiesel (Knothe, 2009)
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