Abstract
The toxicity of Egyptian stabilized rice bran extract (RBE) has been evaluated for acute and repeated 28 day oral toxicity with the outcome of genotoxicity. The extract was administered to female white albino rats at 2000 mg kg-1 for acute testing and to male and female rats at concentrations of 1000, 500, and 100 mg kg-1 for 28 days. Mutagenic potential was evaluated. Chromosomal and DNA damage using standard karyotyping and alkaline comet assay were applied. Acute study showed neither deaths nor gross of pathological abnormalities. In 28 days study, no dose-related changes were observed in body weights, haematological as well as the majority of the serum biochemistry parameters. Results showed neither mutagenic nor genotoxic effects of the RBE. Histological findings showed significant changes in rat groups administering 1000 and 500 mg kg-1 doses. The extract proved to be non toxic acutely and the degree of toxicity was noticed to be dose dependent after chronic administration. Thus we suggest that RBE at dose of 100 mg kg-1 (equivalent to 1 g oil daily consumption) is safe to be administered as dietary supplement for long term use. Key words: Stabilized rice bran oil extract (RBE), ames test, alkaline comet assay, chromosomal aberrations, white albino rats.
Highlights
Rice bran (Oryza sativa) is a by-product of the rice milling industry and comprises of 10% of the whole rice grain.Worldwide production of rice bran has reached about 50 to 60 million metric tons per year
We suggest that rice bran extract (RBE) at dose of 100 mg kg-1 is safe to be administered as dietary supplement for long term use
HPLC finger-print of SRB oil extract γ–Oryzanol content in the RBE sample determined by HPLC was 2.42%
Summary
The purpose of the present study is to investigate the toxicity of the RBE extracted from rice bran after stabilization process
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