Abstract
Bean leaf roll virus (BLRV) is a recently isolated virus from Vicia fabae beans which was collected from eight different locations at Ismailia governorate, Egypt. This virus is belonging to the genus luteovirus and resembles its members in mode of transmission, symptoms and morphological appearance. In this study, BLRV was identified using multi techniques, direct enzyme linked immunosorbent assay (ELISA), antiserum with purified antigen, insect transmission and reverse transcription-polymerase chain reaction (RT-PCR). All plants collected from El- Tall El-Caber farms gave positive reactions with specific BLRV antibodies. Moreover, typical symptoms of BLRV, stunting, leaf roll, mosaic, chlorosis and yellowing, appeared on all plant cultivars under test (Giza 714, Giza 429, Giza461, Giza717 and Giza3). Six aphid species, Acyrthosiphon pisum, Aphis craccivora, Aphis fabae, Aphis gossypii, Macrosiphum euphorbiae and Myzus persicae, have obligatory transmitted BLRV in a circulative persistent manner. The percentages of transmission, acquisition and inoculation feeding period have been determined. RT-PCR technique was used for the detection and identification of the isolated virus particles from the infected faba bean plants. Using specific oligonucleotide primers, the coat protein gene, cp, was amplified from BLRV genome producing 400 bp fragments. Key words: Faba bean plant, Aphis gossypii, Myzus persicae, BLRV, ELISA, RT-PCR.
Highlights
Bean leaf roll virus (BLRV) which is known as pea leaf roll virus, is distributed worldwide (Ashby, 1984)
Samples were inspected for BLRV symptoms and marked for further detection assay by direct enzyme linked immunosorbent assay (ELISA) test, according to the method described by Clark and Adams (1977)
Faba bean plants showing the identical symptoms of leaf roll were grown and reacting positively with BLRV antiserum and healthy faba bean plants were used for further experiments
Summary
Bean leaf roll virus (BLRV) which is known as pea leaf roll virus, is distributed worldwide (Ashby, 1984). Vicia faba L, is considered as one of the most important food legumes in Egypt. The species is considered a major staple food crop that is important for human and animal nutrition in developing countries (Bond, 1987). BLRV is endemic in white clover in the Southeastern United States (Damsteegt et al, 1995). Cool-season food legumes (faba bean, lentil, chickpea and pea) and cereals (bread and durum wheat and barley) are the most important and widely cultivated crops in West Asia and North Africa (WANA), where they are the main source of carbohydrates and protein for the majority of the population. Cool-season food legumes (faba bean, lentil, chickpea and pea) and cereals (bread and durum wheat and barley) are the most important and widely cultivated crops in West Asia and North Africa (WANA), where they are the main source of carbohydrates and protein for the majority of the population. (Makkouk and Kumari, 2009)
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