Abstract
Niger (Guzoita abyssinica) is traditionally very important in the production of oil and as an oilseed crop in Ethiopia. The level of Niger genetic diversity is not studied at molecular level particularly with microsatellite markers which are cost effective, informative and co-dominant. In this study, contrasting samples of 65 Ethiopian Niger germplasm accessions collected from diversified eco-geographic regions were studied with eleven polymorphic EST derived SSR markers. Based on dissimilarity matrix ranging from 0.056 to 0.75, the accessions were grouped into three major and six sub clusters, showing the wealth of genetic diversity for exploitation in future breeding programs. The EST derived SSR markers used in this study also revealed high polymorphic information content (PIC) ranging from 0.2624 to 0.3677, the average being 0.3308 which indicate the usefulness of the primers in Niger germplasm characterization in the future by providing basic breeding information for breeders. The mean number of major allele frequency, gene diversity and heterozygosity using power marker v3.25, showed a value of 0.69, 0.42 and 0.50, respectively. The number of private alleles using GenAlEx 6.41 is 66 alleles with mean of 9 alleles per locus. Thus, utilizing EST SSR marker for diversity study lays basic foundation to understand the genetic distance of crop verities and greatly contributes for further improvement and preservation. Key words: Alleles, dendrogram, diversity, EST-SSR markers, heterozygosity, locus, Niger and polymorphic information content (PIC).
Highlights
Niger (Guzoita abyssinica, 2n=30) is belongs to the plant family “Asteraceae”
The detailed material and methods used to evaluate the genetic diversity of 65 Niger germplasms using 11 EST- SSR markers is clearly described as follows
19 SSR markers were obtained from published articles (Dempewolf et al, 2010a), and screened to select 11 of them which were polymorphic with understandable and solid band for statistics on germplasm, and produced total numbers of alleles with an average of 9 alleles per locus
Summary
Niger (Guzoita abyssinica, 2n=30) is belongs to the plant family “Asteraceae”. It is one of the most valuable oilseed crops in Ethiopia (Getinet and Sharma, 1996). Niger grows in water-logged soils where other oil-seed crops fail, and its cultivation is beneficial for soil conservation and rehabilitation.
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