Diversity Among Finger Millet Accessions Based on Genotyping Potential of SSR, EST-SSR and ISSR Markers

Abstract

Genetic diversity is the basis for adaptability of any organism against environmental changes through natural selection. Microsatellites markers are mostly used for studying population genetic diversity because of their locus-specific and hyper-variability. Availability of sequences (DNA) paved a way for the development of EST-SSRs. The ISSR markers were identified based on the amplification regions; usually 100–3000 bp present between inversely oriented closely spaced microsatellites. 40 SSR markers, 33 EST-SSR markers, 10 ISSR markers were used in the current study to check diversity among 30 diverse finger millet accessions from different geographical regions. 29 SSR markers, 21 EST-SSR markers and 10 ISSR markers exhibited polymorphism in which 19 SSR markers, 13 EST SSR markers and 8 ISSR markers have a PIC value above 0.5. 30 accessions are categorized into four major groups basing on the distribution of their polymorphic alleles. This current study demonstrates the use of different primers in studying diverse finger millet accessions. These identified primers will be valuable sources for any further genetic studies on Finger millet.